BRENDA - Enzyme Database

Geranoyl-CoA carboxylase: a novel biotin-containing enzyme in plants

Guan, X.; Diez, T.; Prasad, T.K.; Nikolau, B.J.; Wurtele, E.S.; Arch. Biochem. Biophys. 362, 12-21 (1999) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.4.1.5
Avidin
complete inhibition, prevented by incubation of avidin with biotin prior to its addition
Zea mays
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.4.1.5
0.0084
-
ATP
-
Zea mays
6.4.1.5
0.064
-
geranoyl-CoA
-
Zea mays
6.4.1.5
0.58
-
HCO3-
-
Zea mays
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.4.1.5
plastid
-
Zea mays
9536
-
6.4.1.5
plastid
84% of GCCase activity is recovered in plastids
Daucus carota
9536
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.4.1.5
Mg2+
absolute requirement
Zea mays
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.4.1.5
122000
-
x * 122000, biotin-containing subunit, SDS-PAGE, Western blot analysis
Zea mays
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
6.4.1.5
ATP + geranoyl-CoA + HCO3-
Zea mays
plays an important role in isoprenoid catabolism
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
Zea mays
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
Glycine max
plays an important role in isoprenoid catabolism
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
Glycine max
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
Daucus carota
plays an important role in isoprenoid catabolism
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
Daucus carota
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
6.4.1.5
Daucus carota
-
-
-
6.4.1.5
Glycine max
-
-
-
6.4.1.5
Zea mays
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
6.4.1.5
184fold, from leaves
Zea mays
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.4.1.5
additional information
-
specific activities in different developing embryos, effect of cotyledon development and cell growth on GCCase activity
Daucus carota
6.4.1.5
additional information
-
specific activities in several tissues of 4 and 15 days old seedlings
Glycine max
6.4.1.5
additional information
-
specific activities in several tissues of 7 days old seedlings
Zea mays
6.4.1.5
0.138
-
-
Zea mays
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
6.4.1.5
ATP + geranoyl-CoA + HCO3-
ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA
649418
Zea mays
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
formation of gamma-carboxygeranoyl-CoA
649418
Zea mays
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA
649418
Glycine max
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
formation of gamma-carboxygeranoyl-CoA
649418
Glycine max
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA
649418
Daucus carota
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
formation of gamma-carboxygeranoyl-CoA
649418
Daucus carota
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
plays an important role in isoprenoid catabolism
649418
Zea mays
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
649418
Zea mays
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
plays an important role in isoprenoid catabolism
649418
Glycine max
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
649418
Glycine max
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
plays an important role in isoprenoid catabolism
649418
Daucus carota
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
649418
Daucus carota
?
6.4.1.5
additional information
not: methylcrotonyl-CoA
649418
Zea mays
?
-
649418
Zea mays
?
Subunits
EC Number
Subunits
Commentary
Organism
6.4.1.5
?
x * 122000, biotin-containing subunit, SDS-PAGE, Western blot analysis
Zea mays
Synonyms
EC Number
Synonyms
Commentary
Organism
6.4.1.5
GCCase
-
Zea mays
6.4.1.5
GCCase
-
Glycine max
6.4.1.5
GCCase
-
Daucus carota
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4.1.5
8.3
-
-
Zea mays
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
6.4.1.5
additional information
-
active over a broad pH-range
Zea mays
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.4.1.5
biotin
biotin-dependent enzyme
Zea mays
6.4.1.5
biotin
biotin-dependent enzyme
Glycine max
6.4.1.5
biotin
biotin-dependent enzyme
Daucus carota
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.4.1.5
biotin
biotin-dependent enzyme
Zea mays
6.4.1.5
biotin
biotin-dependent enzyme
Glycine max
6.4.1.5
biotin
biotin-dependent enzyme
Daucus carota
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.4.1.5
Avidin
complete inhibition, prevented by incubation of avidin with biotin prior to its addition
Zea mays
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.4.1.5
0.0084
-
ATP
-
Zea mays
6.4.1.5
0.064
-
geranoyl-CoA
-
Zea mays
6.4.1.5
0.58
-
HCO3-
-
Zea mays
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.4.1.5
plastid
-
Zea mays
9536
-
6.4.1.5
plastid
84% of GCCase activity is recovered in plastids
Daucus carota
9536
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.4.1.5
Mg2+
absolute requirement
Zea mays
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.4.1.5
122000
-
x * 122000, biotin-containing subunit, SDS-PAGE, Western blot analysis
Zea mays
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
6.4.1.5
ATP + geranoyl-CoA + HCO3-
Zea mays
plays an important role in isoprenoid catabolism
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
Zea mays
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
Glycine max
plays an important role in isoprenoid catabolism
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
Glycine max
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
Daucus carota
plays an important role in isoprenoid catabolism
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
Daucus carota
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.4.1.5
184fold, from leaves
Zea mays
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.4.1.5
additional information
-
specific activities in different developing embryos, effect of cotyledon development and cell growth on GCCase activity
Daucus carota
6.4.1.5
additional information
-
specific activities in several tissues of 4 and 15 days old seedlings
Glycine max
6.4.1.5
additional information
-
specific activities in several tissues of 7 days old seedlings
Zea mays
6.4.1.5
0.138
-
-
Zea mays
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
6.4.1.5
ATP + geranoyl-CoA + HCO3-
ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA
649418
Zea mays
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
formation of gamma-carboxygeranoyl-CoA
649418
Zea mays
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA
649418
Glycine max
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
formation of gamma-carboxygeranoyl-CoA
649418
Glycine max
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA
649418
Daucus carota
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
formation of gamma-carboxygeranoyl-CoA
649418
Daucus carota
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
plays an important role in isoprenoid catabolism
649418
Zea mays
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
649418
Zea mays
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
plays an important role in isoprenoid catabolism
649418
Glycine max
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
649418
Glycine max
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
plays an important role in isoprenoid catabolism
649418
Daucus carota
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
649418
Daucus carota
?
6.4.1.5
additional information
not: methylcrotonyl-CoA
649418
Zea mays
?
-
649418
Zea mays
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.4.1.5
?
x * 122000, biotin-containing subunit, SDS-PAGE, Western blot analysis
Zea mays
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4.1.5
8.3
-
-
Zea mays
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
6.4.1.5
additional information
-
active over a broad pH-range
Zea mays