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Literature summary extracted from

  • Carnall, N.; Webb, H.; Carrington, M.
    Mutagenesis study of the glycosylphosphatidylinositol phospholipase C of Trypanosoma brucei (1997), Mol. Biochem. Parasitol., 90, 423-432.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.14 overexpression in Escherichia coli Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.14 C184S fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 C24S fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 C269S fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 C270S fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 C273S fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 C332S fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 C347S fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 C80S fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 H34Q enzyme is totally inactive Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.14 p-chloromercuriphenylsulfonic acid
-
 Trypanosoma brucei
4.6.1.14 Zn2+
-
 Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.14 0.0004 0.0007 Variant-surface-glycoprotein pH 8 Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.14 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.14 recombinant protein Trypanosoma brucei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.14 0.45
-
-
 Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.14 variant-surface-glycoprotein
-
 Trypanosoma brucei 1,2-didecanoylglycerol + soluble variant-surface-glycoprotein
-
 ?