EC Number | Application | Comment | Organism |
---|---|---|---|
4.3.3.2 | synthesis | immobilized enzyme can be used to synthesize large quantities of 3-alpha(S)-strictosidine, that is a common intermediate in the biosynthesis of indole alkaloids | Catharanthus roseus |
EC Number | General Stability | Organism |
---|---|---|
4.3.3.2 | immobilized enzyme is more stable to changes in pH and temperature than soluble enzyme | Catharanthus roseus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.3.3.2 | tryptamine | inhibitory above 1 mM, soluble and immobilized enzyme | Catharanthus roseus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.3.2 | 0.9 | - |
tryptamine | immobilized enzyme, pH 6.5, 37°C | Catharanthus roseus | |
4.3.3.2 | 2.1 | - |
secologanin | immobilized enzyme, pH 6.5, 37°C | Catharanthus roseus | |
4.3.3.2 | 2.3 | - |
tryptamine | soluble enzyme, pH 6.5, 37°C | Catharanthus roseus | |
4.3.3.2 | 3.4 | - |
secologanin | soluble enzyme, pH 6.5, 37°C | Catharanthus roseus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.2 | tryptamine + secologanin | Catharanthus roseus | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.3.2 | Catharanthus roseus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.3.2 | - |
Catharanthus roseus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.3.3.2 | cell culture | - |
Catharanthus roseus | - |
EC Number | Storage Stability | Organism |
---|---|---|
4.3.3.2 | 4°C, immobilized enzyme, 2 years, 55% loss of activity | Catharanthus roseus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.2 | tryptamine + secologanin | - |
Catharanthus roseus | 3-alpha(S)-strictosidine | - |
? | |
4.3.3.2 | tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Catharanthus roseus | 3-alpha(S)-strictosidine | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.3.3.2 | 37 | - |
immobilized enzyme, half life: 68 d, 6% of initial activity after one year | Catharanthus roseus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.3.3.2 | 6.4 | - |
immobilized enzyme | Catharanthus roseus |
4.3.3.2 | 6.8 | - |
soluble enzyme | Catharanthus roseus |