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Literature summary extracted from

  • Hasler, T.; Curtius, H.C.
    Purification and characterization of 6-pyruvoyl tetrahydropterin synthase from salmon liver (1989), Eur. J. Biochem., 180, 205-211.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.12 dithioerythritol increased catalytic activity with 10 mM Salmo salar

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.12 N-(7-Dimethyl-amino-4-methyl coumarinyl) maleimide
-
 Salmo salar

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.12 0.0022
-
 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
 Salmo salar
4.2.3.12 0.0022
-
 7,8-dihydroneopterin triphosphate pH 7.4, 37ºC Salmo salar

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.12 16000
-
 x * 16000, SDS-PAGE Salmo salar
4.2.3.12 17000
-
 x * 17000, SDS-PAGE Salmo salar
4.2.3.12 68000
-
 gel filtration Salmo salar

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.12 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin Salmo salar one of the enzymes involved in tetrahydrobiopterin biosynthesis 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 7,8-dihydroneopterin triphosphate Salmo salar assay at 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.12 Salmo salar
-
 salmon
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.3.12 additional information free of mannose and glucose residues Salmo salar
4.2.3.12 no glycoprotein no carbohydrates bound Salmo salar

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.12 ammonium sulfate precipitation, hydrophobic interaction chromatography, gel filtration, ion exchange, hydroxyapatite chromatography Salmo salar

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.12 liver
-
 Salmo salar
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.12 4.6
-
-
 Salmo salar

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.12 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
 Salmo salar 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin one of the enzymes involved in tetrahydrobiopterin biosynthesis Salmo salar 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 7,8-dihydroneopterin triphosphate
-
 Salmo salar 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 7,8-dihydroneopterin triphosphate assay at Salmo salar 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.12 ? x * 16000, SDS-PAGE Salmo salar
4.2.3.12 ? x * 17000, SDS-PAGE Salmo salar

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.3.12 80
-
 5 min, 25% loss of activity Salmo salar

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.12 7.5
-
-
 Salmo salar
4.2.3.12 7.5
-
 in Tris-HCl 100 mM Salmo salar