Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jacobson, C.B.; Pasternak, J.J.; Glick, B.R.
    Partial purification and characterization of 1-aminocyclopropane-1-carboxylate deaminase from the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 (1994), Can. J. Microbiol., 40, 1019-1025.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.99.7 cytoplasm
-
 Pseudomonas putida 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.99.7 35000
-
 3 * 35000, SDS-PAGE Pseudomonas putida
3.5.99.7 105000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.99.7 1-aminocyclopropane-1-carboxylate + H2O Pseudomonas putida the enzyme is thought to be intimately involved in the mechanism that the bacterium uses to promote root elongation in developing Canola seedlings. The enzyme is inducible by 1-aminocyclopropane-1-carboxylate and contains a basal level of constitutively expressed enzyme activity 2-oxobutanoate + NH3
-
 ?
3.5.99.7 1-aminocyclopropane-1-carboxylate + H2O Pseudomonas putida GR12-2 the enzyme is thought to be intimately involved in the mechanism that the bacterium uses to promote root elongation in developing Canola seedlings. The enzyme is inducible by 1-aminocyclopropane-1-carboxylate and contains a basal level of constitutively expressed enzyme activity 2-oxobutanoate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.99.7 Pseudomonas putida
-
 GR12-2
-
3.5.99.7 Pseudomonas putida GR12-2
-
 GR12-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.99.7 partial Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.99.7 1-aminocyclopropane-1-carboxylate + H2O
-
 Pseudomonas putida 2-oxobutanoate + NH3
-
 ?
3.5.99.7 1-aminocyclopropane-1-carboxylate + H2O the enzyme is thought to be intimately involved in the mechanism that the bacterium uses to promote root elongation in developing Canola seedlings. The enzyme is inducible by 1-aminocyclopropane-1-carboxylate and contains a basal level of constitutively expressed enzyme activity Pseudomonas putida 2-oxobutanoate + NH3
-
 ?
3.5.99.7 1-aminocyclopropane-1-carboxylate + H2O
-
 Pseudomonas putida GR12-2 2-oxobutanoate + NH3
-
 ?
3.5.99.7 1-aminocyclopropane-1-carboxylate + H2O the enzyme is thought to be intimately involved in the mechanism that the bacterium uses to promote root elongation in developing Canola seedlings. The enzyme is inducible by 1-aminocyclopropane-1-carboxylate and contains a basal level of constitutively expressed enzyme activity Pseudomonas putida GR12-2 2-oxobutanoate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.99.7 trimer 3 * 35000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.99.7 30
-
-
 Pseudomonas putida

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.99.7 15
-
 15°C: about 50% of maximal activity, 40°C: about 35% of maximal activity Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.99.7 8.5
-
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.99.7 7.5 9.5 pH 7.5: about 45% of maximal activity, pH 9.5: 20% of maximal activity Pseudomonas putida