EC Number | General Stability | Organism |
---|---|---|
3.4.17.11 | activity can increase up to 30% if the enzyme at all stages of purification is stored at -20°C | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.17.11 | Ac-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
3.4.17.11 | Ala-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
3.4.17.11 | Benzoyl-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
3.4.17.11 | Benzyloxycarbonyl-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
3.4.17.11 | Benzyloxycarbonyl-Gly-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
3.4.17.11 | Glu-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
3.4.17.11 | Gly-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
3.4.17.11 | His-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
3.4.17.11 | Ile-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
3.4.17.11 | Phe-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
3.4.17.11 | Tyr-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.17.11 | Zn2+ | required | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.17.11 | Pseudomonas sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.17.11 | - |
Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.17.11 | additional information | - |
- |
Pseudomonas sp. |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.17.11 | -196°C, stable for several months | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.17.11 | folic acid + H2O | - |
Pseudomonas sp. | pteroic acid + glutamic acid | - |
? | |
3.4.17.11 | methotrexate + H2O | - |
Pseudomonas sp. | 4-amino-4-deoxy-N10-methylpteroic acid + L-glutamate | - |
? | |
3.4.17.11 | additional information | rapid hydrolysis occurs in linkages of the form RCO-Glu and RCO-Gln, whereas those of the form RCO-D-Glu (D-Glu-D-Glu and Z-D-Glu) are not attacked | Pseudomonas sp. | ? | - |
? | |
3.4.17.11 | additional information | no hydrolysis of glutamate or glutamine lacking a free carboxyl alpha-carboxyl | Pseudomonas sp. | ? | - |
? |