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Literature summary extracted from

  • Yasuhara, T.; Nakai, T.; Ohashi, A.
    Aminopeptidase Y, a new aminopeptidase from Saccharomyces cerevisiae. Purification, properties, localization, and processing by protease B (1994), J. Biol. Chem., 269, 13644-13650.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.15 additional information activation of 75000 Da-proform to 70000 Da active form by protease B Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.15 1,10-phenanthroline 95% inhibition at 1 mM Saccharomyces cerevisiae
3.4.11.15 amastatin 90% inhibition at 0.02 mM Saccharomyces cerevisiae
3.4.11.15 bestatin 48% inhibition at 0.02 mM Saccharomyces cerevisiae
3.4.11.15 Cu2+ 90% inhibition at 0.5 mM Saccharomyces cerevisiae
3.4.11.15 diphosphate 93% inhibition at 1 mM Saccharomyces cerevisiae
3.4.11.15 dithiothreitol 94% inhibition at 1 mM Saccharomyces cerevisiae
3.4.11.15 EDTA 99% inhibition at 1 mM Saccharomyces cerevisiae
3.4.11.15 EGTA 99.9% inhibition at 1 mM Saccharomyces cerevisiae
3.4.11.15 Mn2+ 67% inhibition at 0.5 mM Saccharomyces cerevisiae
3.4.11.15 p-chloromercuribenzoic acid 81% inhibition at 0.1 mM Saccharomyces cerevisiae
3.4.11.15 Triphosphate 99.9% inhibition at 1 mM Saccharomyces cerevisiae
3.4.11.15 Zn2+ 0.81-0.84 mol per mol enzyme; 72% inhibition at 0.5 mM Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.15 autophagic vacuole
-
 Saccharomyces cerevisiae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.15 Co2+ required Saccharomyces cerevisiae
3.4.11.15 Co2+ 800-900% activation by 0.5 mM Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.15 70000
-
 1 * 70000, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.15 Saccharomyces cerevisiae
-
 2 molecular forms
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.15 glycoprotein
-
 Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.15
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.15 additional information
-
 substrate-dependent Saccharomyces cerevisiae
3.4.11.15 0.028
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.15 (Arg)3 + H2O
-
 Saccharomyces cerevisiae Arg + Arg-Arg
-
 ir
3.4.11.15 (Leu)3 + H2O
-
 Saccharomyces cerevisiae Leu + Leu-Leu
-
 ir
3.4.11.15 (Lys)3 + H2O
-
 Saccharomyces cerevisiae Lys + Lys-Lys
-
 ir
3.4.11.15 Ala-7-amido-4-methylcoumarin + H2O
-
 Saccharomyces cerevisiae Ala + 7-amino-4-methylcoumarin
-
 ir
3.4.11.15 Arg-7-amido-4-methylcoumarin + H2O
-
 Saccharomyces cerevisiae Arg + 7-amino-4-methylcoumarin
-
 ir
3.4.11.15 Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Saccharomyces cerevisiae Arg + Arg-4-methylcoumaryl-7-amide
-
 ir
3.4.11.15 Arg-Arg-Lys-Ala-Leu-Ser-Pro + H2O
-
 Saccharomyces cerevisiae Arg + Arg-Lys-Ala-Leu-Ser-Pro
-
 ir
3.4.11.15 Arg-Gly-Tyr-Ser-Leu-Gly + H2O
-
 Saccharomyces cerevisiae Arg + Gly-Tyr-Ser-Leu-Gly
-
 ir
3.4.11.15 Arg-Val + H2O
-
 Saccharomyces cerevisiae Arg + Val
-
 ir
3.4.11.15 Arg-Val-OMe + H2O
-
 Saccharomyces cerevisiae Arg + Val-OMe
-
 ir
3.4.11.15 Arg-Val-Tyr-Ile-His-Pro-Phe + H2O
-
 Saccharomyces cerevisiae Arg + Val-Tyr-Ile-His-Pro-Phe
-
 ir
3.4.11.15 Leu-7-amido-4-methylcoumarin + H2O
-
 Saccharomyces cerevisiae Leu + 7-amino-4-methylcoumarin
-
 ir
3.4.11.15 Leu-Arg-Arg-Ala-Ser-Leu-Gly + H2O
-
 Saccharomyces cerevisiae Leu + Arg-Arg-Ala-Ser-Leu-Gly
-
 ir
3.4.11.15 Leu-Leu + H2O
-
 Saccharomyces cerevisiae Leu + Leu
-
 ir
3.4.11.15 Leu-Leu-OMe + H2O
-
 Saccharomyces cerevisiae Leu + Leu-OMe
-
 ir
3.4.11.15 Lys-7-amido-4-methylcoumarin + H2O
-
 Saccharomyces cerevisiae Lys + 7-amino-4-methylcoumarin
-
 ir
3.4.11.15 Lys-Ala-4-methylcoumaryl-7-amide + H2O
-
 Saccharomyces cerevisiae Lys + Ala-4-methylcoumaryl-7-amide
-
 ir
3.4.11.15 Met-7-amido-4-methylcoumarin + H2O
-
 Saccharomyces cerevisiae Met + 7-amino-4-methylcoumarin
-
 ir
3.4.11.15 Met-Ala-Ser + H2O
-
 Saccharomyces cerevisiae Met + Ala-Ser
-
 ir
3.4.11.15 Met-Arg-Phe + H2O
-
 Saccharomyces cerevisiae Met + Arg-Phe
-
 ir
3.4.11.15 Met-Gly-Gly + H2O
-
 Saccharomyces cerevisiae Met + Gly-Gly
-
 ir
3.4.11.15 additional information generally higher activities with dipeptide-4-methylcoumaryl-7-amides than with amino-acid-4-methylcoumaryl-7-amides Saccharomyces cerevisiae ?
-
 ?
3.4.11.15 Pro-Phe-Arg-4-methylcoumaryl-7-amide + H2O
-
 Saccharomyces cerevisiae Pro + Phe-Arg-4-methylcoumaryl-7-amide
-
 ir
3.4.11.15 Ser-7-amido-4-methylcoumarin + H2O
-
 Saccharomyces cerevisiae Ser + 7-amino-4-methylcoumarin
-
 ir
3.4.11.15 Tyr-Gly-Gly-Phe-Leu + H2O
-
 Saccharomyces cerevisiae Tyr + Gly-Gly-Phe-Leu
-
 ir

Subunits

EC Number Subunits Comment Organism
3.4.11.15 monomer 1 * 70000, SDS-PAGE Saccharomyces cerevisiae