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Literature summary extracted from

  • Riazuddin, S.; Athar, A.; Ahmed, Z.; Lali, S.M.; Sohail, A.
    DNA glycosylase enzymes induced during chemical adaptation of M. luteus (1987), Nucleic Acids Res., 15, 6607-6624.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.2.21 spermidine stimulates activity Escherichia coli
3.2.2.21 spermine stimulates activity Escherichia coli

General Stability

EC Number General Stability Organism
3.2.2.20 very unstable Micrococcus luteus
3.2.2.21 very unstable Micrococcus luteus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.20 3-Methyladenine
-
 Escherichia coli
3.2.2.20 3-Methyladenine
-
 Micrococcus luteus
3.2.2.20 CaCl2
-
 Micrococcus luteus
3.2.2.20 additional information
-
 Escherichia coli
3.2.2.20 additional information unaffected by spermine or spermidine Micrococcus luteus
3.2.2.20 N-ethylmaleimide
-
 Escherichia coli
3.2.2.20 N-ethylmaleimide
-
 Micrococcus luteus
3.2.2.20 p-mercuribenzoate
-
 Micrococcus luteus
3.2.2.21 CaCl2
-
 Micrococcus luteus
3.2.2.21 additional information resistant to product inhibition by free 3-methyladenine Escherichia coli
3.2.2.21 N-ethylmaleimide
-
 Escherichia coli
3.2.2.21 N-ethylmaleimide
-
 Micrococcus luteus
3.2.2.21 NaCl
-
 Escherichia coli
3.2.2.21 p-mercuribenzoate
-
 Micrococcus luteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.20 KCl
-
 Micrococcus luteus
3.2.2.20 Mg2+
-
 Escherichia coli
3.2.2.20 Mg2+ no obligatory requirement, but addition of 1.5 mM MgCl2 causes stimulation in the catalytic activity 2fold Micrococcus luteus
3.2.2.20 NaCl
-
 Micrococcus luteus
3.2.2.21 Mg2+ no obligatory requirement, but addition of 1.5 mM MgCl2 causes stimulation in the catalytic activity 2fold Micrococcus luteus
3.2.2.21 Mg2+ stimulated by 1.5 mM MgCl2 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.20 21100
-
-
 Escherichia coli
3.2.2.20 22000
-
 gel filtration, Svedberg equation Micrococcus luteus
3.2.2.21 22000
-
 gel filtration, Svedberg equation Micrococcus luteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.20 alkylated DNA + H2O Micrococcus luteus cellular repair of alkylated DNA base modifications 3-methyladenine + ?
-
 ?
3.2.2.20 alkylated DNA + H2O Escherichia coli constititively expressed 3-methyladenine + ?
-
 ?
3.2.2.20 alkylated DNA + H2O Micrococcus luteus constititively expressed 3-methyladenine + ?
-
 ?
3.2.2.21 alkylated DNA + H2O Escherichia coli
-
 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
 ?
3.2.2.21 alkylated DNA + H2O Micrococcus luteus cellular repair of alkylated DNA base modifications 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.20 Escherichia coli
-
-
-
3.2.2.20 Micrococcus luteus
-
-
-
3.2.2.21 Escherichia coli
-
-
-
3.2.2.21 Micrococcus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.20
-
 Micrococcus luteus
3.2.2.21
-
 Micrococcus luteus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.20 0.000242
-
-
 Micrococcus luteus
3.2.2.21 0.000375
-
-
 Micrococcus luteus

Storage Stability

EC Number Storage Stability Organism
3.2.2.20 -20°C, stored in 50% glycerol, undergoes complete inactivation in 3-4 weeks Micrococcus luteus
3.2.2.21 -20°C, stored in 50% glycerol, undergoes complete inactivation in 3-4 weeks Micrococcus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.20 alkylated DNA + H2O calf thymus DNA Escherichia coli 3-methyladenine + ?
-
 ?
3.2.2.20 alkylated DNA + H2O calf thymus DNA Micrococcus luteus 3-methyladenine + ?
-
 ?
3.2.2.20 alkylated DNA + H2O cellular repair of alkylated DNA base modifications Micrococcus luteus 3-methyladenine + ?
-
 ?
3.2.2.20 alkylated DNA + H2O constititively expressed Escherichia coli 3-methyladenine + ?
-
 ?
3.2.2.20 alkylated DNA + H2O constititively expressed Micrococcus luteus 3-methyladenine + ?
-
 ?
3.2.2.20 additional information
-
 Escherichia coli ?
-
 ?
3.2.2.20 additional information no activity on O2-methylcytosine, O2-methylthymine, O4-methylthymine or O6-methylguanine Micrococcus luteus ?
-
 ?
3.2.2.21 alkylated DNA + H2O
-
 Escherichia coli 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
 ?
3.2.2.21 alkylated DNA + H2O
-
 Micrococcus luteus 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
 ?
3.2.2.21 alkylated DNA + H2O cellular repair of alkylated DNA base modifications Micrococcus luteus 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
 ?
3.2.2.21 alkylated DNA + H2O
-
 Micrococcus luteus 3-methyladenine + 3-methylguanine + 7-methyladenine + 7-methylguanine + DNA
-
 ?
3.2.2.21 additional information
-
 Escherichia coli ?
-
 ?
3.2.2.21 additional information no activity on O2-methylcytosine, O2-methylthymine, O4-methylthymine or O6-methylguanine Micrococcus luteus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.20 GI
-
 Micrococcus luteus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.2.20 50
-
 5 min time for 50% inactivation Escherichia coli
3.2.2.20 50
-
 1.2 min time for 50% inactivation Micrococcus luteus
3.2.2.20 60
-
 heat labile, during a 2 min incubation reduces activity to less than 2% Micrococcus luteus
3.2.2.21 50
-
 65 min time for 50% inactivation Escherichia coli
3.2.2.21 50
-
 3.5 min, 50% inactivation Micrococcus luteus
3.2.2.21 60
-
 heat stable, retains 40% of its original activity after 2 min incubation Micrococcus luteus

Cofactor

EC Number Cofactor Comment Organism Structure
3.2.2.20 additional information no absolute cofactor requirement Escherichia coli
3.2.2.20 additional information no absolute cofactor requirement Micrococcus luteus
3.2.2.21 additional information no absolute cofactor requirement Micrococcus luteus