EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.113 | Triton X-100 | requirement | Rattus norvegicus | |
3.2.1.113 | Triton X-100 | 0.12%, no activation at 0.07% and below | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.113 | 1-deoxymannojirimycin | reversible | Rattus norvegicus | |
3.2.1.113 | Co2+ | - |
Rattus norvegicus | |
3.2.1.113 | Cu2+ | - |
Rattus norvegicus | |
3.2.1.113 | EDTA | - |
Rattus norvegicus | |
3.2.1.113 | additional information | no inhibition by Ca2+; no inhibition by Mg2+ | Rattus norvegicus | |
3.2.1.113 | p-chloromercuriphenylsulfonic acid | - |
Rattus norvegicus | |
3.2.1.113 | Tris | - |
Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.113 | Golgi membrane | - |
Rattus norvegicus | 139 | - |
3.2.1.113 | membrane | - |
Rattus norvegicus | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.113 | 230000 | - |
PAGE | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.113 | Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O | Rattus norvegicus | - |
(Man)5GlcNAc + 4 D-mannose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.113 | Rattus norvegicus | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.113 | glycoprotein | 0.88% hexoses | Rattus norvegicus |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.113 | mannosidase IA | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.113 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.113 | additional information | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.113 | 0-4°C, in 10 mM potassium phosphate buffer, pH 7.2, 0.5% Triton X-100, 4-6 months | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.113 | Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O | - |
Rattus norvegicus | (Man)5GlcNAc + 4 D-mannose | - |
? | |
3.2.1.113 | Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O | the terminal mannose on the middle antenna appears to be the most susceptible residue | Rattus norvegicus | (Man)5GlcNAc + 4 D-mannose | - |
? | |
3.2.1.113 | Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O | - |
Rattus norvegicus | ? + D-mannose | - |
? | |
3.2.1.113 | Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNA + H2O | - |
Rattus norvegicus | ? + D-mannose | - |
? | |
3.2.1.113 | Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn + H2O | - |
Rattus norvegicus | ? + D-mannose | - |
? | |
3.2.1.113 | Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | without alpha-1,2-linked mannose residues: no substrate | Rattus norvegicus | (Man)4(GlcNAc)2 + (Man)3(GlcNAc)2 + D-mannose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.113 | tetramer | 4 * 57000-58000, SDS-PAGE | Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.113 | 37 | - |
assay at | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.113 | 6 | - |
- |
Rattus norvegicus |