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Literature summary extracted from

  • Broustas, C.G.; Hajra, A.K.
    Purification, properties, and specificity of rat brain cytosolic fatty acyl coenzyme A hydrolase (1995), J. Neurochem., 64, 2345-2353.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.2 bovine serum albumin a small amount activates the hydrolase Rattus norvegicus
3.1.2.20 bovine serum albumin a small amount activates the hydrolase Rattus norvegicus

General Stability

EC Number General Stability Organism
3.1.2.2 30% ethylene glycol, 50% loss of activity after 5 days Rattus norvegicus
3.1.2.2 purified enzyme needs 30% v/v ethylene glycol for stabilization Rattus norvegicus
3.1.2.20 30% ethylene glycol, 50% loss of activity after 5 days Rattus norvegicus
3.1.2.20 purified enzyme needs 30% v/v ethylene glycol for stabilization Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 1-acyl-sn-glycerol-3-phosphocholine competitive inhibition at low concentration of palmitoyl-CoA Rattus norvegicus
3.1.2.2 bovine serum albumin a high amount inhibits the hydrolase Rattus norvegicus
3.1.2.2 Ca2+ 50% inhibition at 1.6 mM Rattus norvegicus
3.1.2.2 diethyldicarbonate inactivation Rattus norvegicus
3.1.2.2 L-palmitoyl carnitine competitive to acyl-CoA Rattus norvegicus
3.1.2.2 lysophosphatidylcholine
-
 Rattus norvegicus
3.1.2.2 lysophosphatidylinositol
-
 Rattus norvegicus
3.1.2.2 methyl methanethiosulfonate slight inhibition Rattus norvegicus
3.1.2.2 Mg2+ 50% inhibition at 1.6 mM Rattus norvegicus
3.1.2.2 additional information no inhibition by NEM, iodoacetamide, L-carnitine Rattus norvegicus
3.1.2.2 octylglucoside 50% inhibition at 0.3% Rattus norvegicus
3.1.2.2 p-hydroxymercuribenzoate
-
 Rattus norvegicus
3.1.2.2 PMSF no inhibition Rattus norvegicus
3.1.2.2 SDS 50% inhibition at 0.035% Rattus norvegicus
3.1.2.2 sodium deoxycholate 50% inhibition at 0.04% Rattus norvegicus
3.1.2.2 Triton X-100 50% inhibition at 0.01% Rattus norvegicus
3.1.2.20 1-acyl-sn-glycerol-3-phosphocholine competitive inhibition at low concentration of palmitoyl-CoA Rattus norvegicus
3.1.2.20 bovine serum albumin a high amount inhibits the hydrolase Rattus norvegicus
3.1.2.20 Ca2+ 50% inhibition at 1.6 mM Rattus norvegicus
3.1.2.20 diethyldicarbonate inactivation Rattus norvegicus
3.1.2.20 L-palmitoyl carnitine competitive to acyl-CoA Rattus norvegicus
3.1.2.20 lysophosphatidylcholine
-
 Rattus norvegicus
3.1.2.20 lysophosphatidylinositol
-
 Rattus norvegicus
3.1.2.20 methyl methanethiosulfonate slight inhibition Rattus norvegicus
3.1.2.20 Mg2+ 50% inhibition at 1.6 mM Rattus norvegicus
3.1.2.20 additional information no inhibition by NEM, iodoacetamide, L-carnitine Rattus norvegicus
3.1.2.20 octylglucoside 50% inhibition at 0.3% Rattus norvegicus
3.1.2.20 p-hydroxymercuribenzoate
-
 Rattus norvegicus
3.1.2.20 PMSF no inhibition Rattus norvegicus
3.1.2.20 SDS 50% inhibition at 0.035% Rattus norvegicus
3.1.2.20 sodium deoxycholate 50% inhibition at 0.04% Rattus norvegicus
3.1.2.20 Triton X-100 50% inhibition at 0.01% Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 0.0038
-
 dodecanoyl-CoA pH 7.5, 37°C Rattus norvegicus
3.1.2.2 0.0046
-
 Arachidonoyl-CoA pH 7.5, 37°C Rattus norvegicus
3.1.2.2 0.0058
-
 hexadecanoyl-CoA pH 7.5, 37°C Rattus norvegicus
3.1.2.20 0.0038
-
 dodecanoyl-CoA pH 7.5, 37°C Rattus norvegicus
3.1.2.20 0.0046
-
 Arachidonoyl-CoA pH 7.5, 37°C Rattus norvegicus
3.1.2.20 0.0058
-
 hexadecanoyl-CoA pH 7.5, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 cytosol
-
 Rattus norvegicus 5829
-
3.1.2.20 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 40900
-
 4 * 40900, SDS-PAGE Rattus norvegicus
3.1.2.2 40900
-
 x * 40900, SDS-PAGE Rattus norvegicus
3.1.2.2 148000
-
 gel filtration Rattus norvegicus
3.1.2.20 40900
-
 4 * 40900, SDS-PAGE Rattus norvegicus
3.1.2.20 40900
-
 x * 40900, SDS-PAGE Rattus norvegicus
3.1.2.20 148000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 hexadecanoyl-CoA + H2O Rattus norvegicus i.e. palmitoyl-CoA CoA + hexadecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O Rattus norvegicus i.e. palmitoyl-CoA CoA + hexadecanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Rattus norvegicus
-
-
-
3.1.2.20 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 3500fold to homogeneity from brain cytosol Rattus norvegicus
3.1.2.20 3500fold to homogeneity from brain cytosol Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.2 brain
-
 Rattus norvegicus
-
3.1.2.20 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 1478
-
 purified enzyme, substrate palmitoyl-CoA Rattus norvegicus
3.1.2.20 1478
-
 purified enzyme, substrate palmitoyl-CoA Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.2.2 -70°C in the presence of 20% glycerol over 1 month Rattus norvegicus
3.1.2.2 -70°C, partially purified enzyme, at least 1 month without loss of activity Rattus norvegicus
3.1.2.2 -70°C, pure enzyme, 0.1 M potassium phosphate, pH 7.5, 30% v/v ethylene glycol, loss of 50% activity within 5 days Rattus norvegicus
3.1.2.20 -70°C in the presence of 20% glycerol over 1 month Rattus norvegicus
3.1.2.20 -70°C, partially purified enzyme, at least 1 month without loss of activity Rattus norvegicus
3.1.2.20 -70°C, pure enzyme, 0.1 M potassium phosphate, pH 7.5, 30% v/v ethylene glycol, loss of 50% activity within 5 days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 acetoacetyl-CoA + H2O 8% activity compared to palmitoyl-CoA Rattus norvegicus CoA + acetoacetate
-
 ?
3.1.2.2 acetyl-CoA + H2O no activity Rattus norvegicus CoA + acetate
-
 ?
3.1.2.2 acyl-CoA + H2O broad specificity for medium-chain to long-chain acyl-CoA Rattus norvegicus CoA + a carboxylate
-
 ?
3.1.2.2 acyl-CoA + H2O saturated and unsaturated acyl-CoA Rattus norvegicus CoA + a carboxylate
-
 ?
3.1.2.2 acyl-CoA + H2O specific for acyl-CoA, S-palmitoyl-glutathione and S-palmitoyl-cysteine are no substrates Rattus norvegicus CoA + a carboxylate
-
 ?
3.1.2.2 arachidonoyl-CoA + H2O i.e. all-cis-5,8,11,14-eicosatetranoate Rattus norvegicus CoA + arachidonate
-
 ?
3.1.2.2 dodecanoyl-CoA + H2O best substrate Rattus norvegicus CoA + dodecanoate
-
 ?
3.1.2.2 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Rattus norvegicus CoA + dodecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Rattus norvegicus CoA + hexadecanoate
-
 ?
3.1.2.2 additional information no activity with malonyl-CoA, ligneceroyl-CoA, and nervonoyl-CoA Rattus norvegicus ?
-
 ?
3.1.2.2 n-butyryl-CoA + H2O no activity Rattus norvegicus CoA + n-butanoate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O
-
 Rattus norvegicus CoA + tetradecanoate
-
 ?
3.1.2.20 acetoacetyl-CoA + H2O 8% activity compared to palmitoyl-CoA Rattus norvegicus CoA + acetoacetate
-
 ?
3.1.2.20 acetyl-CoA + H2O no activity Rattus norvegicus CoA + acetate
-
 ?
3.1.2.20 acyl-CoA + H2O broad specificity for medium-chain to long-chain acyl-CoA Rattus norvegicus CoA + a carboxylate
-
 ?
3.1.2.20 acyl-CoA + H2O saturated and unsaturated acyl-CoA Rattus norvegicus CoA + a carboxylate
-
 ?
3.1.2.20 acyl-CoA + H2O specific for acyl-CoA, S-palmitoyl-glutathione and S-palmitoyl-cysteine are no substrates Rattus norvegicus CoA + a carboxylate
-
 ?
3.1.2.20 arachidonoyl-CoA + H2O i.e. all-cis-5,8,11,14-eicosatetranoate Rattus norvegicus CoA + arachidonate
-
 ?
3.1.2.20 dodecanoyl-CoA + H2O best substrate Rattus norvegicus CoA + dodecanoate
-
 ?
3.1.2.20 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Rattus norvegicus CoA + dodecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Rattus norvegicus CoA + hexadecanoate
-
 ?
3.1.2.20 additional information no activity with malonyl-CoA, ligneceroyl-CoA, and nervonoyl-CoA Rattus norvegicus ?
-
 ?
3.1.2.20 n-butyryl-CoA + H2O no activity Rattus norvegicus CoA + n-butanoate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O
-
 Rattus norvegicus CoA + tetradecanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.2 ? x * 40900, SDS-PAGE Rattus norvegicus
3.1.2.2 tetramer 4 * 40900, SDS-PAGE Rattus norvegicus
3.1.2.20 ? x * 40900, SDS-PAGE Rattus norvegicus
3.1.2.20 tetramer 4 * 40900, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.2 37
-
 assay at Rattus norvegicus
3.1.2.20 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 7.5
-
 assay at Rattus norvegicus
3.1.2.20 7.5
-
 assay at Rattus norvegicus