BRENDA - Enzyme Database

Nonoxidative ethanol metabolism in rabbit myocardium: purification to homogeneity of fatty acyl ethyl ester synthase

Mogelson, S.; Lange, L.G.; Biochemistry 23, 4075-4081 (1984)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.67
1-butanol
at concentrations greater than 5times Km
Oryctolagus cuniculus
3.1.1.67
1-propanol
at concentrations greater than 5times Km
Oryctolagus cuniculus
3.1.1.67
albumin
-
Oryctolagus cuniculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.67
0.2
-
arachidonate
pH 7.2, 37°C, presence of ethanol
Oryctolagus cuniculus
3.1.1.67
0.2
-
linoleate
pH 7.2, 37°C
Oryctolagus cuniculus
3.1.1.67
0.2
-
oleate
pH 7.2, 37°C
Oryctolagus cuniculus
3.1.1.67
0.2
-
palmitate
pH 7.2, 37°C
Oryctolagus cuniculus
3.1.1.67
0.2
-
stearate
pH 7.2, 37°C
Oryctolagus cuniculus
3.1.1.67
70
-
1-butanol
pH 7.2, 37°C, cosubstrate oleate
Oryctolagus cuniculus
3.1.1.67
530
-
1-propanol
pH 7.2, 37°C, cosubstrate oleate
Oryctolagus cuniculus
3.1.1.67
1100
-
ethanol
pH 7.2, 37°C, cosubstrate oleate
Oryctolagus cuniculus
3.1.1.67
1300
-
methanol
pH 7.2, 37°C, cosubstrate oleate, value above
Oryctolagus cuniculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.67
soluble
-
Oryctolagus cuniculus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.67
26000
-
2 * 26000, SDS-PAGE
Oryctolagus cuniculus
3.1.1.67
50000
-
gel filtration under nondenaturing conditions
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.1.1.67
fatty acid + ethanol
Oryctolagus cuniculus
catalyzes fatty acyl ethyl ester synthesis independently of coenzyme A and ATP
fatty acyl ethyl ester + H2O
-
Oryctolagus cuniculus
?
3.1.1.67
fatty acid + ethanol
Oryctolagus cuniculus
production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects
fatty acyl ethyl ester + H2O
-
Oryctolagus cuniculus
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.1.1.67
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.1.1.67
5400fold
Oryctolagus cuniculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.67
heart
ventricular myocardium
Oryctolagus cuniculus
-
3.1.1.67
myocardium
ventricular
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.67
additional information
-
-
Oryctolagus cuniculus
3.1.1.67
0.0112
-
pH 7.2, 37°C
Oryctolagus cuniculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.1.1.67
arachidonate + ethanol
18.3% of the activity with linoleate
646062
Oryctolagus cuniculus
ethyl arachidonate + H2O
-
-
-
?
3.1.1.67
fatty acid + ethanol
enzyme specificity
646062
Oryctolagus cuniculus
fatty acyl ethyl ester + H2O
-
646062
Oryctolagus cuniculus
?
3.1.1.67
fatty acid + ethanol
catalyzes fatty acyl ethyl ester synthesis independently of coenzyme A and ATP
646062
Oryctolagus cuniculus
fatty acyl ethyl ester + H2O
-
646062
Oryctolagus cuniculus
?
3.1.1.67
fatty acid + ethanol
production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects
646062
Oryctolagus cuniculus
fatty acyl ethyl ester + H2O
-
646062
Oryctolagus cuniculus
?
3.1.1.67
linoleate + ethanol
highest activity
646062
Oryctolagus cuniculus
ethyl linoleate + H2O
-
-
-
?
3.1.1.67
additional information
no hydrolysis of cholesteryl oleate
646062
Oryctolagus cuniculus
?
-
-
-
-
3.1.1.67
oleate + 1-butanol
63.2% of the activity with 1-propanol
646062
Oryctolagus cuniculus
1-butyl oleate + H2O
-
-
-
?
3.1.1.67
oleate + 1-propanol
highest activity with 1-propanol
646062
Oryctolagus cuniculus
1-propyl oleate + H2O
-
-
-
?
3.1.1.67
oleate + ethanol
79.1% of the activity with linoleate, 81.6% of the activity with 1-propanol
646062
Oryctolagus cuniculus
ethyl oleate + H2O
-
646062
Oryctolagus cuniculus
?
3.1.1.67
oleate + methanol
74.7% of the activity with 1-propanol
646062
Oryctolagus cuniculus
methyl oleate + H2O
-
-
-
?
3.1.1.67
palmitate + ethanol
7.3% of the activity with linoleate
646062
Oryctolagus cuniculus
ethyl palmitate + H2O
-
-
-
?
3.1.1.67
stearate + ethanol
1.6% of the activity with linoleate
646062
Oryctolagus cuniculus
ethyl stearate + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.67
dimer
2 * 26000, SDS-PAGE
Oryctolagus cuniculus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.67
37
-
assay at
Oryctolagus cuniculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.67
7.2
-
assay at
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.67
1-butanol
at concentrations greater than 5times Km
Oryctolagus cuniculus
3.1.1.67
1-propanol
at concentrations greater than 5times Km
Oryctolagus cuniculus
3.1.1.67
albumin
-
Oryctolagus cuniculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.67
0.2
-
arachidonate
pH 7.2, 37°C, presence of ethanol
Oryctolagus cuniculus
3.1.1.67
0.2
-
linoleate
pH 7.2, 37°C
Oryctolagus cuniculus
3.1.1.67
0.2
-
oleate
pH 7.2, 37°C
Oryctolagus cuniculus
3.1.1.67
0.2
-
palmitate
pH 7.2, 37°C
Oryctolagus cuniculus
3.1.1.67
0.2
-
stearate
pH 7.2, 37°C
Oryctolagus cuniculus
3.1.1.67
70
-
1-butanol
pH 7.2, 37°C, cosubstrate oleate
Oryctolagus cuniculus
3.1.1.67
530
-
1-propanol
pH 7.2, 37°C, cosubstrate oleate
Oryctolagus cuniculus
3.1.1.67
1100
-
ethanol
pH 7.2, 37°C, cosubstrate oleate
Oryctolagus cuniculus
3.1.1.67
1300
-
methanol
pH 7.2, 37°C, cosubstrate oleate, value above
Oryctolagus cuniculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.67
soluble
-
Oryctolagus cuniculus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.67
26000
-
2 * 26000, SDS-PAGE
Oryctolagus cuniculus
3.1.1.67
50000
-
gel filtration under nondenaturing conditions
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.1.1.67
fatty acid + ethanol
Oryctolagus cuniculus
catalyzes fatty acyl ethyl ester synthesis independently of coenzyme A and ATP
fatty acyl ethyl ester + H2O
-
Oryctolagus cuniculus
?
3.1.1.67
fatty acid + ethanol
Oryctolagus cuniculus
production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects
fatty acyl ethyl ester + H2O
-
Oryctolagus cuniculus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.67
5400fold
Oryctolagus cuniculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.67
heart
ventricular myocardium
Oryctolagus cuniculus
-
3.1.1.67
myocardium
ventricular
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.67
additional information
-
-
Oryctolagus cuniculus
3.1.1.67
0.0112
-
pH 7.2, 37°C
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.1.1.67
arachidonate + ethanol
18.3% of the activity with linoleate
646062
Oryctolagus cuniculus
ethyl arachidonate + H2O
-
-
-
?
3.1.1.67
fatty acid + ethanol
enzyme specificity
646062
Oryctolagus cuniculus
fatty acyl ethyl ester + H2O
-
646062
Oryctolagus cuniculus
?
3.1.1.67
fatty acid + ethanol
catalyzes fatty acyl ethyl ester synthesis independently of coenzyme A and ATP
646062
Oryctolagus cuniculus
fatty acyl ethyl ester + H2O
-
646062
Oryctolagus cuniculus
?
3.1.1.67
fatty acid + ethanol
production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects
646062
Oryctolagus cuniculus
fatty acyl ethyl ester + H2O
-
646062
Oryctolagus cuniculus
?
3.1.1.67
linoleate + ethanol
highest activity
646062
Oryctolagus cuniculus
ethyl linoleate + H2O
-
-
-
?
3.1.1.67
additional information
no hydrolysis of cholesteryl oleate
646062
Oryctolagus cuniculus
?
-
-
-
-
3.1.1.67
oleate + 1-butanol
63.2% of the activity with 1-propanol
646062
Oryctolagus cuniculus
1-butyl oleate + H2O
-
-
-
?
3.1.1.67
oleate + 1-propanol
highest activity with 1-propanol
646062
Oryctolagus cuniculus
1-propyl oleate + H2O
-
-
-
?
3.1.1.67
oleate + ethanol
79.1% of the activity with linoleate, 81.6% of the activity with 1-propanol
646062
Oryctolagus cuniculus
ethyl oleate + H2O
-
646062
Oryctolagus cuniculus
?
3.1.1.67
oleate + methanol
74.7% of the activity with 1-propanol
646062
Oryctolagus cuniculus
methyl oleate + H2O
-
-
-
?
3.1.1.67
palmitate + ethanol
7.3% of the activity with linoleate
646062
Oryctolagus cuniculus
ethyl palmitate + H2O
-
-
-
?
3.1.1.67
stearate + ethanol
1.6% of the activity with linoleate
646062
Oryctolagus cuniculus
ethyl stearate + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.67
dimer
2 * 26000, SDS-PAGE
Oryctolagus cuniculus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.67
37
-
assay at
Oryctolagus cuniculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.67
7.2
-
assay at
Oryctolagus cuniculus