EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.8.3.16 | N-ethylmaleimide | 20% inhibition at 1 mM | Oxalobacter formigenes | |
2.8.3.16 | p-chloromercuribenzoate | 91% inhibition at 1 mM, causes precipitation | Oxalobacter formigenes |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.3.16 | 2.3 | - |
succinate | pH 6.7, 25°C | Oxalobacter formigenes | |
2.8.3.16 | 3 | - |
formyl-CoA | pH 6.7, 25°C | Oxalobacter formigenes | |
2.8.3.16 | 5.1 | - |
oxalate | pH 6.7, 25°C | Oxalobacter formigenes |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.8.3.16 | 44000 | - |
1 * 44000, SDS-PAGE | Oxalobacter formigenes |
2.8.3.16 | 44700 | - |
gel filtration | Oxalobacter formigenes |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.3.16 | formyl-CoA + oxalate | Oxalobacter formigenes | - |
formate + oxalyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.3.16 | Oxalobacter formigenes | O06644 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.3.16 | 31.2fold, to homogeneity | Oxalobacter formigenes |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.8.3.16 | 2.15 | - |
purified enzyme | Oxalobacter formigenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.3.16 | formyl-CoA + oxalate | - |
Oxalobacter formigenes | formate + oxalyl-CoA | - |
? | |
2.8.3.16 | additional information | no activity with acetate or malonate | Oxalobacter formigenes | ? | - |
? | |
2.8.3.16 | succinyl-CoA + oxalate | 11% of the reaction rate observed with formyl-CoA | Oxalobacter formigenes | succinate + oxalyl-CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.8.3.16 | monomer | 1 * 44000, SDS-PAGE | Oxalobacter formigenes |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.8.3.16 | 25 | - |
assay at | Oxalobacter formigenes |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.8.3.16 | 6.5 | 7.5 | - |
Oxalobacter formigenes |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.8.3.16 | Oxalobacter formigenes | - |
4.8 | 4.7 |