Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Trevino, R.J.; Hunt, J.; Horowitz, P.M.; Chirgwin, J.M.
    Chinese hamster rhodanese cDNA: activity of the expressed protein is not blocked by a C-terminal extension (1995), Protein Expr. Purif., 6, 693-699.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.1 expression in Escherichia coli Oncorhynchus mykiss

Protein Variants

EC Number Protein Variants Comment Organism
2.8.1.1 additional information
-
 Bos taurus
2.8.1.1 additional information last 5 amino acids replaced by 28 residues of unrelated sequence, no differences to kinetic constants of wild-type enzyme Oncorhynchus mykiss

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.1 5.6
-
 thiosulfate mutant rhodanese Oncorhynchus mykiss
2.8.1.1 7.9
-
 thiosulfate recombinant rhodanese Oncorhynchus mykiss
2.8.1.1 9.2
-
 thiosulfate recombinant rhodanese Bos taurus
2.8.1.1 9.3
-
 thiosulfate liver rhodanese Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.1 33000
-
 1 * 33000, SDS-PAGE Oncorhynchus mykiss
2.8.1.1 33000 34000 hydrophobic column chromatography Oncorhynchus mykiss

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.1 thiosulfate + cyanide Bos taurus
-
 sulfite + thiocyanate
-
 ?
2.8.1.1 thiosulfate + cyanide Oncorhynchus mykiss
-
 sulfite + thiocyanate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.1 Bos taurus
-
-
-
2.8.1.1 Oncorhynchus mykiss
-
 chinese hamster
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.1
-
 Oncorhynchus mykiss

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.1.1 ovary
-
 Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.1 491
-
 C-terminally extended mutant enzyme Oncorhynchus mykiss
2.8.1.1 818
-
 recombinant wild-type Oncorhynchus mykiss

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.1 thiosulfate + cyanide
-
 Bos taurus sulfite + thiocyanate
-
 ?
2.8.1.1 thiosulfate + cyanide
-
 Oncorhynchus mykiss sulfite + thiocyanate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.1.1 monomer
-
 Bos taurus
2.8.1.1 monomer 1 * 33000, SDS-PAGE Oncorhynchus mykiss