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Literature summary extracted from

  • Lacourciere, G.M.; Stadtman, T.C.
    Catalytic properties of selenophosphate synthetases: comparison of the selenocysteine-containing enzyme from Haemophilus influenzae with the corresponding cysteine-containing enzyme from Escherichia coli (1999), Proc. Natl. Acad. Sci. USA, 96, 44-48.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.3 overexpression in Escherichia coli strain BL21 Haemophilus influenzae

General Stability

EC Number General Stability Organism
2.7.9.3 concentration-dependent inactivation Haemophilus influenzae
2.7.9.3 concentration-dependent inactivation Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.9.3 H2O2 62% inhibition at 10 mM Escherichia coli
2.7.9.3 H2O2 53% inhibition at 5 mM Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.9.3 0.02
-
 selenide pH 7.8, 37°C Escherichia coli
2.7.9.3 0.025
-
 selenide pH 7.8, 37°C Haemophilus influenzae
2.7.9.3 0.9
-
 ATP pH 7.8, 37°C Escherichia coli
2.7.9.3 1.3
-
 ATP pH 7.8, 37°C Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.3 Escherichia coli
-
 gene selD
-
2.7.9.3 Haemophilus influenzae
-
 gene selD
-
2.7.9.3 Haemophilus influenzae
-
 selenocysteine containing enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.3 recombinant from Escherichia coli, to homogeneity Haemophilus influenzae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.9.3 ATP + selenide + H2O = AMP + selenophosphate + phosphate enzyme contains a selenocysteine residue at the active site Haemophilus influenzae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.9.3 16
-
-
 Haemophilus influenzae
2.7.9.3 29
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.3 ATP + selenide + H2O
-
 Haemophilus influenzae AMP + selenophosphate + phosphate
-
 ?
2.7.9.3 ATP + selenide + H2O
-
 Escherichia coli AMP + selenophosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.9.3 37
-
 assay at Haemophilus influenzae
2.7.9.3 37
-
 assay at Escherichia coli