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Literature summary extracted from

  • McAllister, K.A.; Peery, R.B.; Meier, T.I.; Fischl, A.S.; Zhao, G.
    Biochemical and molecular analyses of the Streptococcus pneumoniae acyl carrier protein synthase, an enzyme essential for fatty acid biosynthesis (2000), J. Biol. Chem., 275, 30864-30872.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.7 apo-acyl-carrier protein significantly stimulating above 0.01 mM Streptomyces pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.7
-
 Streptomyces pneumoniae
2.7.8.7 genes acpS and acpP cloned and overexpressed in Escherichia coli, acpS complements an Escherichia coli mutant Streptomyces pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.7 3',5'-ADP product inhibition Streptomyces pneumoniae
2.7.8.7 apo-acyl-carrier protein slight inhibition at 0.005-0.01 mM Streptomyces pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.7 0.0005
-
 apo-acyl-carrier protein pH 7.0, 37°C Streptomyces pneumoniae
2.7.8.7 0.0013
-
 apo-acyl-carrier protein pH 7.0, 37°C, trichloroacetic acid precipitation method Streptomyces pneumoniae
2.7.8.7 0.0071
-
 CoA pH 7.0, 37°C, trichloroacetic acid precipitation method Streptomyces pneumoniae
2.7.8.7 0.0115
-
 CoA pH 7.0, 37°C Streptomyces pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.7 10400
-
 3 * 10400, homotrimer, SDS-PAGE Streptomyces pneumoniae
2.7.8.7 13390
-
 AcpS, calculated from predicted amino acid sequence Streptomyces pneumoniae
2.7.8.7 13390
-
 AcpS, mass spectrometric analysis Streptomyces pneumoniae
2.7.8.7 38000
-
 purified AcpS, gel filtration Streptomyces pneumoniae
2.7.8.7 39000
-
 AcpS, sedimentation analysis Streptomyces pneumoniae
2.7.8.7 41000
-
 AcpS, native homotrimer, predicted from nucleotide sequence Streptomyces pneumoniae
2.7.8.7 53000
-
 gel filtration Streptomyces pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein] Streptomyces pneumoniae transfer of acyl fatty acid intermediates during biosynthesis of fatty acids and lipids in the cell adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.7 Streptomyces pneumoniae
-
-
-
2.7.8.7 Streptomyces pneumoniae P0A2W7 apcS gene, open reading frame starting at the second Met codon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.7 recombinant enzyme Streptomyces pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.7 acetoacetyl-CoA + apo-[acyl-carrier protein]
-
 Streptomyces pneumoniae ? + holo-[acyl-carrier protein]
-
 r
2.7.8.7 acetyl-CoA + apo-[acyl-carrier protein]
-
 Streptomyces pneumoniae CoA + acetyl-[acyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[acyl-carrier protein]
-
 Streptomyces pneumoniae adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[acyl-carrier protein] transfer of acyl fatty acid intermediates during biosynthesis of fatty acids and lipids in the cell Streptomyces pneumoniae adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 desulfo-CoA + apo-[acyl-carrier protein]
-
 Streptomyces pneumoniae ? + holo-[acyl-carrier protein]
-
 r
2.7.8.7 malonyl-CoA + apo-[acyl-carrier protein]
-
 Streptomyces pneumoniae ? + holo-[acyl-carrier protein]
-
 r
2.7.8.7 additional information dephospho-CoA is no substrate Streptomyces pneumoniae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.7 trimer 3 * 13390, homotrimer, mass spectrometric analysis Streptomyces pneumoniae
2.7.8.7 trimer 3 * 10400, homotrimer, SDS-PAGE Streptomyces pneumoniae

Synonyms

EC Number Synonyms Comment Organism
2.7.8.7 AcpS
-
 Streptomyces pneumoniae
2.7.8.7 type II fatty acid synthase system
-
 Streptomyces pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.7 45 50
-
 Streptomyces pneumoniae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.8.7 22 65 stable in this range Streptomyces pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.7 6.5
-
-
 Streptomyces pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.8.7 0.006
-
 3',5'-ADP pH 7.0, 37°C Streptomyces pneumoniae