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Literature summary extracted from

  • Rosenow, C.; Roberts, I.S.; Jann, K.
    Isolation from recombinant Escherichia coli and characterization of CMP-Kdo synthetase, involved in the expression of the capsular K5 polysaccharide (K-CKS) (1995), FEMS Microbiol. Lett., 125, 159-164.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.38 kps gene cluster cloned and sequenced, K-CKS overexpressed in recombinant Escherichia coli JA221 (pCR3) Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.38 2
-
 3-deoxy-D-manno-octulosonate pH 9.5, 30°C Escherichia coli
2.7.7.38 2.5
-
 CTP pH 9.5, 30°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.38 cytoplasm
-
 Escherichia coli 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.38 Mg2+ isoenzymes K-CKS and L-CKS, enzymatic activity critically dependent on magnesium ions Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.38 27000
-
 recombinant KpsU protein, K-CKS, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate Escherichia coli involved in the expression of the capsular K5 polysaccharide K-CKS diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.38 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.38 recombinant KpsU protein, K-CKS Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.38 3300
-
 K-CKS Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate
-
 Escherichia coli diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 r
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate involved in the expression of the capsular K5 polysaccharide K-CKS Escherichia coli diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.7.38 CKS
-
 Escherichia coli
2.7.7.38 K-CKS CKS involved in capsule expression Escherichia coli
2.7.7.38 K-KDO-CT present in pathogenic strains, synthesizes a capsule as response to temperature shifts Escherichia coli
2.7.7.38 L-CKS CKS involved in lipopolysaccharide biosynthesis Escherichia coli
2.7.7.38 L-KDO-CT involved in lipopolysaccharide biosynthesis Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.38 9.5
-
 K-CKS Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.38 Escherichia coli K-CKS, isolectric capillary electrophoresis
-
 3.7