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Literature summary extracted from

  • Marshall, A.D.; Darbyshire, J.F.; McPhie, P.; Jakoby, W.B.
    A review of the effects of manipulation of the cysteine residues of rat aryl sulfotransferase IV (1998), Chem. Biol. Interact., 109, 107-116.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.9 5,5'-dithiobis(2-nitrobenzoate) low concentrations lead to increase of activity at a physiological pH, higher concentrations lead to complete inactivation Rattus norvegicus
2.8.2.9 Diamide low concentrations lead to increase of activity at a physiological pH, higher concentrations lead to complete inactivation Rattus norvegicus
2.8.2.9 glutathione disulfide leads to the formation of internal disulfide bonds between Cys66 and Cys232, activates the enzyme and allows it to function at a pH optimum in the physiological range Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.9
-
 Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.8.2.9 C232S mutant is catalytically active Rattus norvegicus
2.8.2.9 C66S mutant is catalytically active, can not be activated by glutathione disulfide Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.9 Phenylarsine oxide effective inhibitor Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.9 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.9 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.9 3'-phosphoadenylylsulfate + 2-naphthol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + 4-nitrophenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.9 additional information
-
 pH optima for sulfation are dependent on the oxidation state of the sulfotransferase Rattus norvegicus
2.8.2.9 5.4
-
-
 Rattus norvegicus
2.8.2.9 6
-
 two optima: pH 6 and pH 9.7, reduced enzyme activity with 2-naphthylamine as substrate Rattus norvegicus
2.8.2.9 8
-
 oxidized enzyme Rattus norvegicus
2.8.2.9 9.7
-
 two optima: pH 6 and pH 9.7, reduced activity with 2-naphthylamine as substrate Rattus norvegicus