EC Number | Cloned (Comment) | Organism |
---|---|---|
1.7.2.1 | D92E and D92N mutant enzyme | Achromobacter xylosoxidans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.7.2.1 | D92E | mutation in type 2 Cu center, very low activity with artificial electron donors methyl viologen and sodium dithionite, 20-30% of wild-type activity with physiological electron donor azurin I | Achromobacter xylosoxidans |
1.7.2.1 | D92N | mutation in type 2 Cu center, very low activity with artificial electron donors methyl viologen and sodium dithionite, 60-70% of wild-type activity with physiological electron donor azurin I | Achromobacter xylosoxidans |
1.7.2.1 | H139A | mutation in type 1 Cu center, very low activity with the artificial electron donor methyl viologen, no activity with the physiological electron donor azurin I | Achromobacter xylosoxidans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.7.2.1 | copper | the type 1 Cu center functions as electron acceptor from the physiological electron donor azurin I, the type 2 Cu center is involved in binding and reduction of the substrate | Achromobacter xylosoxidans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.2.1 | reduced azurin + O2 | Achromobacter xylosoxidans | putative physiological electron donor | oxidized azurin + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.2.1 | Achromobacter xylosoxidans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.7.2.1 | recombinant wild-type and D92E and D92N mutant enzyme | Achromobacter xylosoxidans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.7.2.1 | 45.2 | - |
native recombinant enzyme, azurin | Achromobacter xylosoxidans |
1.7.2.1 | 107 | - |
native recombinant enzyme, dithionite | Achromobacter xylosoxidans |
1.7.2.1 | 168 | - |
native recombinant enzyme, methyl viologen | Achromobacter xylosoxidans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.2.1 | nitrite + ferrocytochrome c | - |
Achromobacter xylosoxidans | nitric oxide + H2O + ferricytochrome c | - |
? | |
1.7.2.1 | NO2- + reduced azurin | putative physiological electron donor | Achromobacter xylosoxidans | NO + oxidized azurin | - |
? | |
1.7.2.1 | reduced azurin + O2 | putative physiological electron donor | Achromobacter xylosoxidans | oxidized azurin + H2O | - |
? |