Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lee, J.Y.; Park, H.S.; Kim, H.S.
    Adenosylcobalamin-mediated methyl transfer by toluate cis-dihydrodiol dehydrogenase of the TOL plasmid pWW0 (1999), J. Bacteriol., 181, 2953-2957.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.25 expression of xylL gene in Escherichia coli strain from TOL plasmid Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ Pseudomonas sp.
-
 catechol + CO2 + NADH
-
 ?
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ Pseudomonas putida
-
 catechol + CO2 + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.25 Pseudomonas putida
-
 only TOL plasmid is mentioned, which is derived from Pseudomonas putida, but not Pseudomonas putida itself
-
1.3.1.25 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.25 recombinant from Escherichia coli Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
-
 Pseudomonas sp. catechol + CO2 + NADH
-
 ?
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
-
 Pseudomonas putida catechol + CO2 + NADH
-
 ?
1.3.1.25 3-methyl-4,5-cyclohexadiene-cis-1,2-diol-1-carboxylic acid + NAD+
-
 Pseudomonas putida 3-methylcatechol + CO2 + NADH
-
 ?
1.3.1.25 additional information purified recombinant enzyme also shows methyl transfer activity, methyl group is transferred from 4-methyl-3,5-cyclohexadiene-cis-1,2-diol-1-carboxylic acid to 3-methyl, only in presence of adenosylcobalamin as methyl group acceptor Pseudomonas putida ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.25 adenosylcobalamin required as methyl acceptor for the methyl transfer activity of recombinant enzyme from E. coli Pseudomonas putida
1.3.1.25 NAD+
-
 Pseudomonas sp.
1.3.1.25 NAD+
-
 Pseudomonas putida