EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.2 | deoxycholate | stimulates at low subsolubilizing concentrations, membrane-solubilizing concentrations lead to nearly complete inactivation | Rattus norvegicus | |
2.7.8.2 | lysolecithin | stimulates at low subsolubilizing concentrations, membrane-solubilizing concentrations lead to nearly complete inactivation | Rattus norvegicus | |
2.7.8.2 | Triton X-100 | stimulates at low subsolubilizing concentrations, membrane-solubilizing concentrations lead to nearly complete inactivation | Rattus norvegicus | |
2.7.8.2 | Tween-20 | stimulates at low subsolubilizing concentrations, membrane-solubilizing concentrations lead to nearly complete inactivation | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.2 | Ca2+ | above 0.01 mM; competitive to Mg2+ or Mn2+ | Rattus norvegicus | |
2.7.8.2 | CMP | inhibition of the reaction CDP-choline + 1,2-diacylglycerol | Rattus norvegicus | |
2.7.8.2 | deoxycholate | stimulates at low subsolubilizing concentrations, membrane-solubilizing concentrations lead to nearly complete inactivation | Rattus norvegicus | |
2.7.8.2 | Triton X-100 | - |
Rattus norvegicus | |
2.7.8.2 | Tween-20 | stimulates at low subsolubilizing concentrations, membrane-solubilizing concentrations lead to nearly complete inactivation | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.8.2 | 0.18 | 0.35 | CMP | 37°C, pH 8.5 | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.8.2 | endoplasmic reticulum | - |
Rattus norvegicus | 5783 | - |
2.7.8.2 | Golgi apparatus | - |
Rattus norvegicus | 5794 | - |
2.7.8.2 | membrane | nuclear membrane, integral membrane protein | Rattus norvegicus | 16020 | - |
2.7.8.2 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.2 | Mg2+ | maximal activation at 5-10 mM | Rattus norvegicus | |
2.7.8.2 | Mn2+ | can partially replace Mg2+ in activation | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.2 | CDP-choline + sn-1,2-diacylglycerol | Rattus norvegicus | final reaction in synthesis of phosphatidylcholine | ? | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.8.2 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.8.2 | partial | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.2 | CDP-choline + 1,2-diacylglycerol | - |
Rattus norvegicus | CMP + a phosphatidylcholine | - |
r | |
2.7.8.2 | CDP-choline + sn-1,2-diacylglycerol | final reaction in synthesis of phosphatidylcholine | Rattus norvegicus | ? | - |
r | |
2.7.8.2 | CMP + phosphatidylcholine | - |
Rattus norvegicus | CDP-choline + 1,2-diacylglycerol | - |
r |