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Literature summary extracted from

  • Varin, L.; Ibrahim, R.K.
    Partial purification and characterization of three flavonol-specific sulfotransferases from Flaveria chloraefolia (1989), Plant Physiol., 90, 977-981.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.25 additional information EDTA, SH-reagents at 1 and 10 mM, e.g. p-chloromercuribenzoate, iodoacetate or iodoacetamide, are not inhibitory Flaveria chlorifolia
2.8.2.26 additional information EDTA up to 10 mM, or SH-group reagents, e.g. p-chloromercuribenzoate, iodoacetate, iodoacetamide at 1 and 10 mM, are not inhibitory Flaveria chlorifolia
2.8.2.27 additional information EDTA up to 10 mM, SH-group reagents, e.g. p-chloromercuribenzoate, iodoacetate, iodoacetamide, all at 1 and 10 mM, are not inhibitory Flaveria chlorifolia
2.8.2.27 phosphate buffer complete inhibition Flaveria chlorifolia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.25 0.0002
-
 quercetin 30°C, pH 7.5 Flaveria chlorifolia
2.8.2.25 0.0002
-
 3'-phosphoadenylylsulfate cosubstrate quercetin, pH 7.5, 30°C Flaveria chlorifolia
2.8.2.26 0.00029
-
 quercetin 3-sulfate
-
 Flaveria chlorifolia
2.8.2.26 0.00035
-
 3'-phosphoadenylylsulfate
-
 Flaveria chlorifolia
2.8.2.27 0.00036
-
 quercetin 3-sulfate 30°C, pH 7.5 Flaveria chlorifolia
2.8.2.27 0.00038
-
 3'-phosphoadenylylsulfate 30°C, pH 7.5 Flaveria chlorifolia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.25 additional information no divalent cation required Flaveria chlorifolia
2.8.2.27 additional information no requirement for divalent cations Flaveria chlorifolia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.25 35000
-
 gel filtration Flaveria chlorifolia
2.8.2.26 35000
-
 gel filtration Flaveria chlorifolia
2.8.2.27 35000
-
 gel filtration Flaveria chlorifolia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.25 3'-phosphoadenylylsulfate + quercetin Flaveria chlorifolia
-
 adenosine 3',5'-bisphosphate + quercetin 3-sulfate
-
 ?
2.8.2.25 additional information Flaveria chlorifolia involved in biosynthesis of polysulfated flavonols ?
-
 ?
2.8.2.26 3'-phosphoadenylylsulfate + quercetin 3-sulfate Flaveria chlorifolia
-
 adenosine 3',5'-bisphosphate + quercetin 3,3'-bissulfate
-
 ?
2.8.2.27 3'-phosphoadenylylsulfate + quercetin 3-sulfate Flaveria chlorifolia
-
 quercetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.25 Flaveria chlorifolia
-
-
-
2.8.2.26 Flaveria chlorifolia
-
-
-
2.8.2.27 Flaveria chlorifolia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.25 partial Flaveria chlorifolia
2.8.2.26 partial Flaveria chlorifolia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.25 shoot tip
-
 Flaveria chlorifolia
-
2.8.2.26 shoot tip
-
 Flaveria chlorifolia
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.25 0.00027
-
-
 Flaveria chlorifolia
2.8.2.26 0.0025
-
-
 Flaveria chlorifolia
2.8.2.27 0.002
-
 30°C, pH 7.5 Flaveria chlorifolia

Storage Stability

EC Number Storage Stability Organism
2.8.2.26 -20°C, 1 mg/ml bovine serum albumin, half-life of 3 days Flaveria chlorifolia
2.8.2.26 4°C, half-life of 24 h Flaveria chlorifolia
2.8.2.27 -20°C, bovine serum albumin up to 1 mg/ml, half-life of 3 days Flaveria chlorifolia
2.8.2.27 4°C, half-life of 24 h Flaveria chlorifolia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.25 3'-phosphoadenylylsulfate + isorhamnetin 94% of activity compared to rhamnetin Flaveria chlorifolia adenosine 3',5'-bisphosphate + isorhamnetin 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + kaempferol 48% of activity compared to rhamnetin Flaveria chlorifolia adenosine 3',5'-bisphosphate + kaempferol 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + ombuin 37% of activity compared to rhamnetin Flaveria chlorifolia adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + patuletin 52% of activity compared to rhamnetin Flaveria chlorifolia adenosine 3',5'-bisphosphate + patuletin 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + quercetin
-
 Flaveria chlorifolia adenosine 3',5'-bisphosphate + quercetin 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + quercetin 58% of activity compared to rhamnetin Flaveria chlorifolia adenosine 3',5'-bisphosphate + quercetin 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + rhamnetin best substrate Flaveria chlorifolia adenosine 3',5'-bisphosphate + rhamnetin 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + tamarixetin 31% of activity compared to rhamnetin Flaveria chlorifolia adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.25 additional information quercetagetin, gossypetin, myricetin or galangin are no substrates Flaveria chlorifolia ?
-
 ?
2.8.2.25 additional information involved in biosynthesis of polysulfated flavonols Flaveria chlorifolia ?
-
 ?
2.8.2.26 3'-phosphoadenylylsulfate + patuletin 3-sulfate 33% of activity compared to quercetin 3-sulfate Flaveria chlorifolia adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.26 3'-phosphoadenylylsulfate + quercetin 3-sulfate
-
 Flaveria chlorifolia adenosine 3',5'-bisphosphate + quercetin 3,3'-bissulfate
-
 ?
2.8.2.26 3'-phosphoadenylylsulfate + tamarixetin 3-sulfate 33% of activity compared to quercetin 3-sulfate Flaveria chlorifolia adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.26 additional information kaempferol 3-sulfate, isorhamnetin 3-sulfate or other flavonol aglycones are no substrates Flaveria chlorifolia ?
-
 ?
2.8.2.27 3'-phosphoadenylylsulfate + isorhamnetin 3-sulfate 38% of activity compared to quercetin 3-sulfate Flaveria chlorifolia isorhamnetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
-
 ?
2.8.2.27 3'-phosphoadenylylsulfate + kaempferol 3-sulfate 45% of activity compared to quercetin 3-sulfate Flaveria chlorifolia kaempferol 3,4'-disulfate + adenosine 3',5'-bisphosphate
-
 ?
2.8.2.27 3'-phosphoadenylylsulfate + patuletin 3-sulfate 12% of activity compared to quercetin 3-sulfate Flaveria chlorifolia patuletin 3,4'-disulfate + adenosine 3',5'-bisphosphate
-
 ?
2.8.2.27 3'-phosphoadenylylsulfate + quercetin 3-sulfate
-
 Flaveria chlorifolia quercetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
-
 ?
2.8.2.27 additional information tamarixetin 3-sulfate, flavonol aglycones, or adenosine 5'-phosphosulfate are no substrates Flaveria chlorifolia ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.25 30
-
 assay at Flaveria chlorifolia
2.8.2.27 30
-
 assay at Flaveria chlorifolia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.25 6.5
-
 and pH 8.5, two maxima Flaveria chlorifolia
2.8.2.25 8.5
-
 and pH 6.5, two maxima Flaveria chlorifolia
2.8.2.26 7.5
-
-
 Flaveria chlorifolia
2.8.2.27 7.5
-
-
 Flaveria chlorifolia