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Literature summary extracted from
- Identification of the gene encoding sulfopyruvate decarboxylase, an enzyme involved in biosynthesis of coenzyme M (2000), J. Bacteriol., 182, 4862-4867.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.1.79 | expression in Escherichia coli | Methanocaldococcus jannaschii |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.79 | 0.64 | - |
sulfopyruvate | - |
Methanocaldococcus jannaschii |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.79 | 17000 | - |
alpha6beta6, 6 * 17000 + 6 * 23000, SDS-PAGE | Methanocaldococcus jannaschii |
| 4.1.1.79 | 23000 | - |
alpha6beta6, 6 * 17000 + 6 * 23000, SDS-PAGE | Methanocaldococcus jannaschii |
| 4.1.1.79 | 210000 | - |
gel filtration | Methanocaldococcus jannaschii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.79 | 3-sulfopyruvate | Methanocaldococcus jannaschii | fourth step in biosynthesis of coenzyme M | 2-sulfoacetaldehyde + CO2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.79 | Methanocaldococcus jannaschii | P58415 | subunitb alpha, P58416, subunit beta | - |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 4.1.1.79 | inactivation by oxygen at 80°C and reactivation by reduction with dithionite | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.79 | 3-sulfopyruvate | - |
Methanocaldococcus jannaschii | 2-sulfoacetaldehyde + CO2 | - |
? | |
| 4.1.1.79 | 3-sulfopyruvate | fourth step in biosynthesis of coenzyme M | Methanocaldococcus jannaschii | 2-sulfoacetaldehyde + CO2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.1.79 | dodecamer | alpha6beta6, 6 * 17000 + 6 * 23000, SDS-PAGE | Methanocaldococcus jannaschii |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.79 | thiamine diphosphate | required | Methanocaldococcus jannaschii | |
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