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Literature summary extracted from

  • Eikmanns, U.; Buckel, W.
    Properties of 5-hydroxyvalerate CoA-transferase from Clostridium aminovalericum (1990), Biol. Chem. Hoppe-Seyler, 371, 1077-1082.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.3.14 ADP slightly Anaerocolumna aminovalerica
2.8.3.14 ATP
-
 Anaerocolumna aminovalerica
2.8.3.14 CTP
-
 Anaerocolumna aminovalerica
2.8.3.14 GTP slightly Anaerocolumna aminovalerica
2.8.3.14 additional information not inhibited by AMP Anaerocolumna aminovalerica
2.8.3.14 Sodium boranate only in presence of a CoA substrate Anaerocolumna aminovalerica
2.8.3.14 UTP slightly Anaerocolumna aminovalerica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.3.14 0.0038
-
 5-hydroxypentanoyl-CoA pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate Anaerocolumna aminovalerica
2.8.3.14 0.009
-
 propionyl-CoA pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate Anaerocolumna aminovalerica
2.8.3.14 0.018
-
 acetyl-CoA pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate Anaerocolumna aminovalerica
2.8.3.14 0.029
-
 butyryl-CoA pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate Anaerocolumna aminovalerica
2.8.3.14 0.03
-
 (Z)-5-hydroxypent-2-enoyl-CoA pH 8.0, 25°C, cosubstrate acetate Anaerocolumna aminovalerica
2.8.3.14 0.037
-
 pentanoyl-CoA pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate Anaerocolumna aminovalerica
2.8.3.14 0.07
-
 butyryl-CoA pH 8.0, 25°C, cosubstrate acetate Anaerocolumna aminovalerica
2.8.3.14 0.1
-
 5-hydroxypentanoyl-CoA pH 8.0, 25°C, cosubstrate acetate Anaerocolumna aminovalerica
2.8.3.14 0.12
-
 propionyl-CoA pH 8.0, 25°C, acetate Anaerocolumna aminovalerica
2.8.3.14 0.45
-
 pentanoyl-CoA pH 8.0, 25°C, cosubstrate acetate Anaerocolumna aminovalerica
2.8.3.14 4
-
 (Z)-5-hydroxy-2-pentenoate pH 8.0, 25°C, cosubstrate acetyl-CoA Anaerocolumna aminovalerica
2.8.3.14 20
-
 acetate pH 8.0, 25°C, cosubstrate propionyl-CoA Anaerocolumna aminovalerica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.14 47000
-
 4 * 47000, homotetramer, SDS-PAGE Anaerocolumna aminovalerica
2.8.3.14 155000 160000 gradient PAGE Anaerocolumna aminovalerica
2.8.3.14 178000
-
 average molecular mass Anaerocolumna aminovalerica
2.8.3.14 180000 195000 gel filtration, Superose 6 Anaerocolumna aminovalerica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.14 5-hydroxypentanoyl-CoA + acetate Anaerocolumna aminovalerica
-
 acetyl-CoA + 5-hydroxypentanoate
-
 ?
2.8.3.14 5-hydroxypentanoyl-CoA + acetate Anaerocolumna aminovalerica T2-7
-
 acetyl-CoA + 5-hydroxypentanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.14 Anaerocolumna aminovalerica
-
 T2-7
-
2.8.3.14 Anaerocolumna aminovalerica T2-7
-
 T2-7
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.14
-
 Anaerocolumna aminovalerica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.14 38 46
-
 Anaerocolumna aminovalerica

Storage Stability

EC Number Storage Stability Organism
2.8.3.14 -20°C, no loss of activity observed after storage for several months Anaerocolumna aminovalerica
2.8.3.14 4°C, lose 50% of its activity within 2 months when kept in 20 mM potassium phosphate buffer, pH 6.8 Anaerocolumna aminovalerica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.14 5-hydroxypentanoyl-CoA + acetate
-
 Anaerocolumna aminovalerica acetyl-CoA + 5-hydroxypentanoate
-
 ?
2.8.3.14 5-hydroxypentanoyl-CoA + acetate
-
 Anaerocolumna aminovalerica T2-7 acetyl-CoA + 5-hydroxypentanoate
-
 ?
2.8.3.14 acetate + (Z)-5-hydroxypent-2-enoyl-CoA
-
 Anaerocolumna aminovalerica acetyl-CoA + (Z)-5-hydroxypent-2-enoate
-
 ?
2.8.3.14 acetyl-CoA + (Z)-5-hydroxy-2-pentenoate
-
 Anaerocolumna aminovalerica acetate + (Z)-5-hydroxy-2-pentenoyl-CoA
-
 r
2.8.3.14 acetyl-CoA + 3-pentenoate
-
 Anaerocolumna aminovalerica acetate + 3-pentenoyl-CoA
-
 ?
2.8.3.14 acetyl-CoA + 4-pentenoate
-
 Anaerocolumna aminovalerica acetate + 4-pentenoyl-CoA
-
 ?
2.8.3.14 acetyl-CoA + 5-hydroxypentanoate
-
 Anaerocolumna aminovalerica acetate + 5-hydroxypentanoyl-CoA
-
 r
2.8.3.14 acetyl-CoA + acetate
-
 Anaerocolumna aminovalerica acetyl-CoA + acetate
-
 ?
2.8.3.14 acetyl-CoA + acetate
-
 Anaerocolumna aminovalerica T2-7 acetyl-CoA + acetate
-
 ?
2.8.3.14 acetyl-CoA + propionate
-
 Anaerocolumna aminovalerica acetate + propanoyl-CoA
-
 r
2.8.3.14 butyryl-CoA + (Z)-5-hydroxy-2-pentenoate
-
 Anaerocolumna aminovalerica butyrate + (Z)-5-hydroxy-2-pentenoyl-CoA
-
 ?
2.8.3.14 butyryl-CoA + acetate
-
 Anaerocolumna aminovalerica acetyl-CoA + butanoate
-
 ?
2.8.3.14 butyryl-CoA + acetate
-
 Anaerocolumna aminovalerica T2-7 acetyl-CoA + butanoate
-
 ?
2.8.3.14 additional information all substrates are bond in a (Z)-2,3-conformation to the enzyme, crotonate, (E)-5-hydroxy-2-pentenoate, (E)-2-pentenoate and 2,4-pentadienoate are no substrates Anaerocolumna aminovalerica ?
-
 ?
2.8.3.14 additional information all substrates are bond in a (Z)-2,3-conformation to the enzyme, crotonate, (E)-5-hydroxy-2-pentenoate, (E)-2-pentenoate and 2,4-pentadienoate are no substrates Anaerocolumna aminovalerica T2-7 ?
-
 ?
2.8.3.14 pentanoyl-CoA + (Z)-5-hydroxy-2-pentenoate
-
 Anaerocolumna aminovalerica pentanoate + (Z)-5-hydroxy-2-pentenoyl-CoA
-
 ?
2.8.3.14 pentanoyl-CoA + acetate
-
 Anaerocolumna aminovalerica acetyl-CoA + pentanoate
-
 ?
2.8.3.14 propionyl-CoA + (Z)-5-hydroxy-2-pentenoate
-
 Anaerocolumna aminovalerica propionate + (Z)-5-hydroxy-2-pentenoyl-CoA
-
 ?
2.8.3.14 propionyl-CoA + acetate
-
 Anaerocolumna aminovalerica acetyl-CoA + propanoate
-
 r
2.8.3.14 propionyl-CoA + acetate
-
 Anaerocolumna aminovalerica T2-7 acetyl-CoA + propanoate
-
 r

Subunits

EC Number Subunits Comment Organism
2.8.3.14 tetramer 4 * 47000, homotetramer, SDS-PAGE Anaerocolumna aminovalerica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.3.14 3
-
 ATP pH 8.0, 25°C substrate acetate, non-competitive inhibition Anaerocolumna aminovalerica
2.8.3.14 3
-
 ATP pH 8.0, 25°C substrate propionyl-CoA, competitive inhibition Anaerocolumna aminovalerica
2.8.3.14 5
-
 ATP pH 8.0, 25°C substrate 5-hydroxypentanoyl-CoA, competitive inhibition Anaerocolumna aminovalerica