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Literature summary extracted from

  • Glendening, T.M.; Poulton, J.E.
    Partial purification and characterization of a 3'-phosphoadenosine 5'-phosphosulfate: desulfoglucosinolate sulfotransferase from cress (Lepidium sativum) (1990), Plant Physiol., 94, 811-816.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.8.2.24 bovine serum albumin, required for stabilizing Lepidium sativum
2.8.2.24 dilution inactivates Lepidium sativum
2.8.2.24 MgCl2, 2-mercaptoethanol, DTT and GSH do not stabilize Lepidium sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.24 2,2'-dipyridyl 26% inhibition at 10 mM Lepidium sativum
2.8.2.24 adenosine-3',5'-bisphosphate product inhibition Lepidium sativum
2.8.2.24 Ag+ 13% inhibition at 1 mM Lepidium sativum
2.8.2.24 Cu2+ 14% inhibition at 1 mM Lepidium sativum
2.8.2.24 DTNB 2-mercaptoethanol protects Lepidium sativum
2.8.2.24 EDTA 15% inhibition at 10 mM Lepidium sativum
2.8.2.24 iodoacetamide weak inhibition Lepidium sativum
2.8.2.24 iodoacetic acid weak inhibition Lepidium sativum
2.8.2.24 additional information no inhibition by FeCl3, FeCl2, CaCl2, CoCl2 Lepidium sativum
2.8.2.24 N-ethylmaleimide 2-mercaptoethanol protects Lepidium sativum
2.8.2.24 Ni2+ 30% inhibition at 1 mM Lepidium sativum
2.8.2.24 o-phenanthroline 34% inhibition at 10 mM Lepidium sativum
2.8.2.24 p-chloromercuriphenylsulfonic acid 2-mercaptoethanol protects Lepidium sativum
2.8.2.24 Pb(NO3)2 25% inhibition at 1 mM Lepidium sativum
2.8.2.24 Zn2+ 46% inhibition at 1 mM Lepidium sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.24 additional information
-
additional information kinetic studies Lepidium sativum
2.8.2.24 0.0065
-
desulfoallylglucosinolate pH 9.0, 30┬░C Lepidium sativum
2.8.2.24 0.06
-
3'-phosphoadenylylsulfate pH 9.0, 30┬░C Lepidium sativum
2.8.2.24 0.082
-
desulfobenzylglucosinolate pH 9.0, 30┬░C Lepidium sativum
2.8.2.24 0.67
-
desulfo-p-hydroxy-benzylglucosinolate pH 9.0, 30┬░C Lepidium sativum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.24 Mg2+ 51% activation at 10 mM Lepidium sativum
2.8.2.24 Mn2+ 23% activation at 1 mM, 10 mM inhibits by 53% Lepidium sativum
2.8.2.24 additional information not affected by FeCl3, FeCl2, CaCl2, CoCl2 Lepidium sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.24 31000
-
gel filtration Lepidium sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.24 3'-phosphoadenylylsulfate + desulfobenzylglucosinolate Lepidium sativum
-
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
2.8.2.24 additional information Lepidium sativum involved in the biosynthesis of glucosinolates ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.24 Arabidopsis thaliana
-
-
-
2.8.2.24 Brassica rapa subsp. oleifera
-
-
-
2.8.2.24 Lepidium sativum
-
no.5089, curled cress
-
2.8.2.24 Sinapis alba
-
-
-
2.8.2.24 Tropaeolum majus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.24 40.1fold Lepidium sativum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.2.24 3'-phosphoadenylyl sulfate + desulfoglucotropeolin = adenosine 3',5'-bisphosphate + glucotropeolin mechanism Lepidium sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.24 seedling etiolated Arabidopsis thaliana
-
2.8.2.24 seedling etiolated Lepidium sativum
-
2.8.2.24 seedling etiolated Brassica rapa subsp. oleifera
-
2.8.2.24 seedling etiolated Sinapis alba
-
2.8.2.24 seedling etiolated Tropaeolum majus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
2.8.2.24 0.935
-
purified enzyme Lepidium sativum

Storage Stability

EC Number Storage Stability Organism
2.8.2.24 -20┬░C, 0.25 M sucrose and bovine serum albumin, 6 months Lepidium sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.24 3'-phosphoadenylyl sulfate + desulfo-p-hydroxy-benzylglucosinolate
-
Lepidium sativum adenosine 3',5'-bisphosphate + p-hydroxy-benzylglucosinolate
-
?
2.8.2.24 3'-phosphoadenylyl sulfate + desulfoallylglucosinolate
-
Lepidium sativum adenosine 3',5'-bisphosphate + allylglucosinolate
-
?
2.8.2.24 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate i.e. 3'-phosphoadenosine 5'phosphosulfate, i.e. PAPS Lepidium sativum adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
2.8.2.24 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Arabidopsis thaliana adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
2.8.2.24 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Lepidium sativum adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
2.8.2.24 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Brassica rapa subsp. oleifera adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
2.8.2.24 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Sinapis alba adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
2.8.2.24 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Tropaeolum majus adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
2.8.2.24 3'-phosphoadenylylsulfate + desulfobenzylglucosinolate
-
Lepidium sativum adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
2.8.2.24 additional information no acceptors are quercetin, rutin, kaempferol, p-coumaric acid, ferulic acid, caffeic acid, phenylacetaldoxime Lepidium sativum ?
-
?
2.8.2.24 additional information involved in the biosynthesis of glucosinolates Lepidium sativum ?
-
?

Subunits

EC Number Subunits Comment Organism
2.8.2.24 monomer 1 * 31000-55000, SDS-PAGE Lepidium sativum

Synonyms

EC Number Synonyms Comment Organism
2.8.2.24 ST
-
Lepidium sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.24 9
-
maximal activity in Tris-HCl buffer Lepidium sativum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.2.24 7 9.5 about 65% of maximal activity at pH 7.0 and pH 9.5 Lepidium sativum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.8.2.24 additional information
-
activity loss due to instability at pH values near the isoelectric point 5.2 Lepidium sativum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.2.24 Lepidium sativum
-
-
5.2