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Literature summary extracted from

  • Iglesias, A.A.; Charng, Y.Y.; Ball, S.; Preiss, J.
    Characterization of the kinetic, regulatory, and structural properties of ADP-glucose pyrophosphorylase from Chlamydomonas reinhardtii (1994), Plant Physiol., 104, 1287-1294.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate allosteric activation Chlamydomonas reinhardtii
2.7.7.27 3-phosphoglycerate activation Chlamydomonas reinhardtii
2.7.7.27 D-fructose 1,6-bisphosphate
-
 Chlamydomonas reinhardtii
2.7.7.27 D-fructose 6-phosphate activation Chlamydomonas reinhardtii
2.7.7.27 D-fructose 6-phosphate slight activation Chlamydomonas reinhardtii
2.7.7.27 D-Glucose 1,6-bisphosphate slight activation Chlamydomonas reinhardtii
2.7.7.27 D-glucose 6-phosphate activation Chlamydomonas reinhardtii
2.7.7.27 D-glucose 6-phosphate slight activation Chlamydomonas reinhardtii
2.7.7.27 NADPH activation Chlamydomonas reinhardtii
2.7.7.27 NADPH activation, slight Chlamydomonas reinhardtii
2.7.7.27 phosphoenolpyruvate activation Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 ADP 1 mM Chlamydomonas reinhardtii
2.7.7.27 diphosphate substrate inhibition, 1 mM Chlamydomonas reinhardtii
2.7.7.27 phosphate allosteric inhibitor Chlamydomonas reinhardtii
2.7.7.27 Rabbit antiserum raised against spinach enzyme not antiserum against E. coli enzyme Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 0.22
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C, kinetic study Chlamydomonas reinhardtii
2.7.7.27 0.48
-
 ATP pH 8.0, 37°C, kinetic study Chlamydomonas reinhardtii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.27 Mg2+ requirement Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.27 50000
-
 x * 50000 + x * 53000 SDS-PAGE Chlamydomonas reinhardtii
2.7.7.27 53000
-
 x * 50000 + x * 53000 SDS-PAGE Chlamydomonas reinhardtii
2.7.7.27 210000
-
 gel filtration Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Chlamydomonas reinhardtii key regulatory enzyme of starch biosynthesis diphosphate + ADP-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Chlamydomonas reinhardtii
-
 green alga, strain 137C
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27
-
 Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 81
-
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Chlamydomonas reinhardtii diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate key regulatory enzyme of starch biosynthesis Chlamydomonas reinhardtii diphosphate + ADP-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.27 heterotetramer x * 50000 + x * 53000 SDS-PAGE Chlamydomonas reinhardtii
2.7.7.27 homotetramer
-
 Chlamydomonas reinhardtii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.27 60
-
 5 min, inactivation Chlamydomonas reinhardtii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.27 0.054
-
 phosphate pH 8.0, 37°C, in absence of 3-phosphoglycerate Chlamydomonas reinhardtii
2.7.7.27 0.2
-
 phosphate pH 8.0, 37°C, in presence of 0.25 mM 3-phosphoglycerate Chlamydomonas reinhardtii
2.7.7.27 0.53
-
 phosphate pH 8.0, 37°C, in presence of 2.5 mM 3-phosphoglycerate Chlamydomonas reinhardtii