Literature summary extracted from
Chowdhury, R.R.
Purification & some properties of galactose-1-phosphate uridylyl transferase from E. coli (1979), Indian J. Biochem. Biophys., 16, 273-277.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
2.7.7.12 |
2-mercaptoethanol |
2fold at 10 mM |
Escherichia coli |
|
2.7.7.12 |
cysteine |
activation |
Escherichia coli |
|
2.7.7.12 |
cysteine |
requirement |
Escherichia coli |
|
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.7.7.12 |
DTNB |
binds to SH-group in the active center |
Escherichia coli |
|
2.7.7.12 |
NEM |
complete inhibition at 0.05 mM |
Escherichia coli |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.7.7.12 |
41000 |
- |
2 * 41000 |
Escherichia coli |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.7.12 |
Escherichia coli |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.7.12 |
over 200fold |
Escherichia coli |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
2.7.7.12 |
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose |
SH-group in the active site |
Escherichia coli |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.7.12 |
UDP-glucose + alpha-D-galactose 1-phosphate |
- |
Escherichia coli |
alpha-D-glucose 1-phosphate + UDP-galactose |
- |
r |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.7.7.12 |
dimer |
2 * 41000 |
Escherichia coli |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.7.7.12 |
8.5 |
- |
- |
Escherichia coli |