Literature summary extracted from
Arnvig, K.; Hove-Jensen, B.; Switzer, R.L.
Purification and properties of phosphoribosyl-diphosphate synthetase from Bacillus subtilis (1990), Eur. J. Biochem., 192, 195-200.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
2.7.6.1 |
phosphate |
requirement |
Bacillus subtilis |
|
2.7.6.1 |
phosphate |
the optimum concentration at pH 8.2 is 50-60 mM, higher concentrations are inhibitory |
Bacillus subtilis |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.6.1 |
expression in Escherichia coli |
Bacillus subtilis |
General Stability
EC Number |
General Stability |
Organism |
---|
2.7.6.1 |
relatively stable in absence of phosphate |
Bacillus subtilis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.7.6.1 |
ADP |
the most effective |
Bacillus subtilis |
|
2.7.6.1 |
AMP |
68% inhibition at 5 mM |
Bacillus subtilis |
|
2.7.6.1 |
ATP |
moderate inhibition above 3 mM |
Bacillus subtilis |
|
2.7.6.1 |
GDP |
64% inhibition at 5 mM |
Bacillus subtilis |
|
2.7.6.1 |
GMP |
88% inhibition at 5 mM |
Bacillus subtilis |
|
2.7.6.1 |
L-histidine |
no inhibition |
Bacillus subtilis |
|
2.7.6.1 |
L-tryptophan |
no inhibition |
Bacillus subtilis |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.7.6.1 |
0.48 |
- |
D-ribose 5-phosphate |
pH 8.2, 37ºC |
Bacillus subtilis |
|
2.7.6.1 |
0.66 |
- |
ATP |
pH 8.2, 37ºC |
Bacillus subtilis |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.7.6.1 |
Mg2+ |
requirement |
Bacillus subtilis |
|
2.7.6.1 |
Mg2+ |
required to form a complex with ATP and as a free cation |
Bacillus subtilis |
|
2.7.6.1 |
Mg2+ |
the most effective |
Bacillus subtilis |
|
2.7.6.1 |
Mn2+ |
can partially replace Mg2+ |
Bacillus subtilis |
|
2.7.6.1 |
Mn2+ |
25-30% of the activity with Mg2+ |
Bacillus subtilis |
|
2.7.6.1 |
additional information |
absolute requirement for a divalent cation for activity |
Bacillus subtilis |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.7.6.1 |
34000 |
- |
8 * 34000, SDS-PAGE |
Bacillus subtilis |
2.7.6.1 |
280000 |
- |
gel filtration |
Bacillus subtilis |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.7.6.1 |
ATP + D-ribose 5-phosphate |
Bacillus subtilis |
the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan |
AMP + 5-phospho-alpha-D-ribose 1-diphosphate |
- |
r |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.6.1 |
Bacillus subtilis |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.6.1 |
streptomycin-heat treatment, ammonium sulfate fractionation, chromatography on DEAE-Sepharose and affinity chromatography |
Bacillus subtilis |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
2.7.6.1 |
ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate |
sequential kinetic mechanism |
Bacillus subtilis |
|
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.7.6.1 |
190 |
- |
30fold to 50fold more activity in the cloned enzyme than in the enzyme from the wild-type cells |
Bacillus subtilis |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
2.7.6.1 |
-70ºC, glycerol 10%, stable |
Bacillus subtilis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.6.1 |
ATP + D-ribose 5-phosphate |
- |
Bacillus subtilis |
AMP + 5-phospho-alpha-D-ribose 1-diphosphate |
- |
r |
|
2.7.6.1 |
ATP + D-ribose 5-phosphate |
the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan |
Bacillus subtilis |
AMP + 5-phospho-alpha-D-ribose 1-diphosphate |
- |
r |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.7.6.1 |
octamer |
8 * 34000, SDS-PAGE |
Bacillus subtilis |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.7.6.1 |
8 |
8.5 |
- |
Bacillus subtilis |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
2.7.6.1 |
7 |
9.5 |
at pH 7 and pH 9.5, about 35% and 85% of the maximal activity, respectively |
Bacillus subtilis |