Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Berger, A.; Schiltz, E.; Schultz, G.E.
    Guanylate kinase from Saccharomyces cerevisiae. Isolation and characterization, crystallization and preliminary X-ray analysis, amino acid sequence and comparison with adenylate kinases (1989), Eur. J. Biochem., 184, 433-443.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.8 octahedral bipyramids, preliminary X-ray analysis Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.8 Mg2+
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.8 20000
-
 gel filtration Saccharomyces cerevisiae
2.7.4.8 20548
-
 1 * 20548, calculated from amino acid sequence Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.8 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.8 method that includes DEAE-Sephacel, Cibacron-blue Sepharose and two Sephadex-75 chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.8 571
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.7.4.8 -30°C, in N-ethylmorpholine-HCl buffer, pH 7.5, several years Saccharomyces cerevisiae
2.7.4.8 4°C, in N-ethylmorpholine-HCl buffer, pH 7.5, several years Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.8 ATP + dGMP
-
 Saccharomyces cerevisiae ADP + dGDP
-
 r
2.7.4.8 ATP + GMP best substrates Saccharomyces cerevisiae ADP + GDP
-
 ?
2.7.4.8 ATP + GMP AMP is no acceptor substrate Saccharomyces cerevisiae ADP + GDP
-
 ?
2.7.4.8 dATP + dGMP
-
 Saccharomyces cerevisiae dADP + dGDP
-
 ?
2.7.4.8 dATP + GMP
-
 Saccharomyces cerevisiae dADP + GDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.8 monomer 1 * 20548, calculated from amino acid sequence Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.8 25
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.8 7.7
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.8 6.8 8.6 about 80% of maximal activity at pH 6.8 and 8.6, about 95% of maximal activity at pH 7.3 and 8 Saccharomyces cerevisiae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.4.8 Saccharomyces cerevisiae pH 7.7, 25ºC
-
 5.8