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Literature summary extracted from

  • Ramotar, K.; Pickard, M.A.
    AMP metabolism by the marine bacterium Vibrio (Benecka) natriegens: purification and properties of adenylate kinase (1981), Can. J. Microbiol., 27, 1053-1059.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.4.3 dithiothreitol and 2-mercaptoethanol are not necessary as protecting agents Vibrio natriegens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.3 EDTA
-
 Vibrio natriegens
2.7.4.3 IAA
-
 Vibrio natriegens
2.7.4.3 N-ethylmaleimide weak Vibrio natriegens
2.7.4.3 p-chloromercuriphenylsulfonate
-
 Vibrio natriegens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.3 0.067
-
 ATP 30°C, pH 7.4 Vibrio natriegens
2.7.4.3 0.114 0.13 AMP
-
 Vibrio natriegens
2.7.4.3 0.45 0.55 ADP 30°C, pH 7.4 Vibrio natriegens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.3 cytosol
-
 Vibrio natriegens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.3 Mg2+ requirement Vibrio natriegens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.3 29000
-
 gel filtration Vibrio natriegens
2.7.4.3 32000
-
 1 * 32000, SDS-PAGE Vibrio natriegens

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Vibrio natriegens
-
 Beneckea, type III
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.3
-
 Vibrio natriegens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.3 1450
-
 30°C, pH 7.4 Vibrio natriegens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 ADP + ADP
-
 Vibrio natriegens ATP + AMP
-
 r
2.7.4.3 ATP + AMP no substrates are ATP/IMP Vibrio natriegens ADP + ADP
-
 r
2.7.4.3 ATP + AMP no substrates are ATP/UMP Vibrio natriegens ADP + ADP
-
 r
2.7.4.3 ATP + AMP no substrates are ATP/GMP Vibrio natriegens ADP + ADP
-
 r
2.7.4.3 ATP + dAMP reaction at 46% the rate of AMP Vibrio natriegens ADP + dADP
-
 ?
2.7.4.3 dATP + AMP reaction at about 50% the rate of ATP Vibrio natriegens dADP + ADP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.3 monomer 1 * 32000, SDS-PAGE Vibrio natriegens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.3 30
-
 assay at Vibrio natriegens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.3 325
-
 ATP
-
 Vibrio natriegens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.3 7 7.6 broad Vibrio natriegens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.3 5 10 less than 20% of maximal activity at pH 5, 5.5, 6 and pH 9.5 and 10, about half-maximal activity at pH 6.9 and 8.4 Vibrio natriegens
2.7.4.3 6.9 8.4 about half-maximal activity at pH 6.9 and 8.4 Vibrio natriegens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.4.3 Vibrio natriegens isoelectric focusing 4.8 4.7