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Literature summary extracted from

  • Thomas, T.M.; Scopes, R.K.
    The effects of temperature on the kinetics and stability of mesophilic and thermophilic 3-phosphoglycerate kinases (1998), Biochem. J., 330, 1087-1095.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.3 expression in Escherichia coli Zymomonas mobilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 additional information
-
 additional information temperature-dependence of the kinetics Zymomonas mobilis
2.7.2.3 additional information
-
 additional information temperature-dependence of the kinetics Thermoanaerobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 42000
-
 gel filtration Thermoanaerobacter sp.
2.7.2.3 44000
-
 gel filtration Zymomonas mobilis

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Thermoanaerobacter sp.
-
-
-
2.7.2.3 Thermoanaerobacter sp.
-
 formerly Thermoanaerobium brockii strain Rt8.G4
-
2.7.2.3 Thermoanaerobacter sp. Rt8.G4
-
-
-
2.7.2.3 Zymomonas mobilis
-
 ATCC 29191
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3
-
 Thermoanaerobacter sp.
2.7.2.3 native from Zymomonas mobilis, and recombinant from Escherichia coli Zymomonas mobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Zymomonas mobilis ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Thermoanaerobacter sp. ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Thermoanaerobacter sp. Rt8.G4 ADP + 1,3-diphosphoglycerate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.3 63
-
-
 Zymomonas mobilis
2.7.2.3 70
-
-
 Thermoanaerobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.3 additional information
-
 thermal analysis Zymomonas mobilis
2.7.2.3 additional information
-
 thermal analysis Thermoanaerobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.3 additional information
-
 additional information temperature-dependence of kinetic Zymomonas mobilis
2.7.2.3 additional information
-
 additional information temperature-dependence of kinetic Thermoanaerobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.3 5.5
-
-
 Thermoanaerobacter sp.
2.7.2.3 6
-
 assay at Zymomonas mobilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Zymomonas mobilis
2.7.2.3 ADP
-
 Thermoanaerobacter sp.
2.7.2.3 ATP required as phosphate donor Zymomonas mobilis
2.7.2.3 ATP required as phosphate donor Thermoanaerobacter sp.