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Literature summary extracted from

  • Macdonald, F.D.; Preiss, J.
    The subcellular location and characteristics of pyrophosphate-D-fructose-6-phosphate 1-phosphotransferase from suspension cultured cells of soybean (1986), Planta, 167, 240-245.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.90 D-fructose 2,6-bisphosphate dependent on substrate concentrations Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.90 diphosphate
-
 Glycine max
2.7.1.90 phosphate
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.90 additional information
-
 additional information kinetic parameters, in the absence or presence of D-fructose 2,6-bisphosphate Glycine max
2.7.1.90 3.8 10.6 D-fructose 6-phosphate pH 7.5, 25°C Glycine max
2.7.1.90 3.8 10.6 D-fructose 6-phosphate with diphosphate Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.90 cytosol
-
 Glycine max 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.90 Mg2+ requirement Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.90 128000
-
 sucrose density gradient centrifugation Glycine max
2.7.1.90 183000
-
 gel filtration Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate Glycine max
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.90 Glycine max
-
 cv. Mandarin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.90 partial, 102fold Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.90 cell suspension culture
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.90 8.47
-
 partially purified enzyme Glycine max

Storage Stability

EC Number Storage Stability Organism
2.7.1.90 -90°C, quick frozen in a dry-ice and methanol bath, at least 6 months Glycine max
2.7.1.90 0°C, in HEPES-glycol buffer, several days Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Glycine max phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Glycine max phosphate + D-fructose 1,6-bisphosphate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.90 25
-
 assay at Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.90 7.5
-
-
 Glycine max