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Literature summary extracted from

  • Iwashima, A.; Nishino, H.; Nose, Y.
    Conversion of thiamine to thiamine monophosphate by cell-free extracts of Escherichia coli (1972), Biochim. Biophys. Acta, 258, 333-336.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.89 ATP dependent on the presence of Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.89 0.0028
-
 thiamine at 37°C, pH 7.5 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.89 soluble in the soluble fraction Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.89 K+ marked stimulation Escherichia coli
2.7.1.89 Mg2+ absolute requirement Escherichia coli
2.7.1.89 NH4+ marked stimulation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.89 ATP + thiamine Escherichia coli
-
 ADP + thiamine monophosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.89 Escherichia coli
-
 K12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.89 partial Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.89 ATP + thiamine
-
 Escherichia coli ADP + thiamine monophosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.89 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.89 7.5
-
 assay at Escherichia coli