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Literature summary extracted from

  • Moritz, A.; Westerman, J.; De Graan, P.N.E.; Wirtz, K.W.A.
    Phosphatidylinositol 4-kinase and phosphatidylinositol-4-phosphate 5-kinase from bovine brain membranes (1992), Methods Enzymol., 209, 202-211.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.68 sucrose and PEG 20000 in the assay medium are required to stabilize the enzyme during phosphorylation reaction Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.68 0.005
-
 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Bos taurus
2.7.1.68 0.019
-
 ATP
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.67 membrane
-
 Bos taurus 16020
-
2.7.1.68 membrane
-
 Bos taurus 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.67 Bos taurus
-
-
-
2.7.1.68 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.67
-
 Bos taurus
2.7.1.68
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.67 brain
-
 Bos taurus
-
2.7.1.68 brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.68 0.171
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.1.68 -20°C, 50 MM Tris-HCl, pH 8.0, 1 mM EDTA, 0.25 M sucrose, 0.3% v/v 2-mercaptoethanol, 0.1% Triton X-100, 0.1% PEG 20000, 0.05 mM ATP, 0.1 mM PMSF, stable for several months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.67 2'-dATP + 1-phosphatidyl-1D-myo-inositol
-
 Bos taurus 2'-dADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?
2.7.1.67 ATP + 1-phosphatidyl-1D-myo-inositol
-
 Bos taurus ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?
2.7.1.67 GTP + 1-phosphatidyl-1D-myo-inositol
-
 Bos taurus GDP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?
2.7.1.68 ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Bos taurus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?