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Literature summary extracted from

  • Prota, A.; Vogt, J.; Pilger, B.; Perozzo, R.; Wurth, C.; Marquez, V.E.; Russ, P.; Schulz, G.E.; Folkers, G.; Scapozza, L.
    Kinetics and crystal structure of the wild-type and the engineered Y101F mutant of Herpes simplex virus type 1 thymidine kinase interacting with (North)-methanocarba-thymidine (2000), Biochemistry, 39, 9597-9603.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.21
-
 Herpes simplex virus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.21 sitting and hanging drop vapor diffusion method Herpes simplex virus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.21 Y101F 12fold increased Km for thymidine Herpes simplex virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.21 2'-exo-methanocarba-thymidine
-
 Herpes simplex virus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 0.0002
-
 thymidine pH 7.2, wild-type Herpes simplex virus
2.7.1.21 0.0025
-
 thymidine pH 7.2, Y101F mutant Herpes simplex virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Herpes simplex virus
-
 type 1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21
-
 Herpes simplex virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + 2'-exo-methanocarba-thymidine MCT Herpes simplex virus ADP + 2'-exo-methanocarba-thymidine 5'-monophosphate
-
 ?
2.7.1.21 ATP + thymidine
-
 Herpes simplex virus ADP + dTMP
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.21 0.011
-
 2'-exo-methanocarba-thymidine pH 7.2, wild-type Herpes simplex virus
2.7.1.21 0.0515
-
 2'-exo-methanocarba-thymidine pH 7.2, Y101F mutant Herpes simplex virus