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Literature summary extracted from
- Valine, not methionine, is amino acid 106 in human cytosolic thymidine kinase (TK1). Impact on oligomerization, stability, and kinetic properties (2000), J. Biol. Chem., 275, 32187-32192.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.7.1.21 | - |
Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 2.7.1.21 | V106M | results in tetrameric structure with high thymidine affinity | Homo sapiens |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.7.1.21 | stable in the presence of ATP, bovine serum albumin and CHAPS | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.7.1.21 | 0.0005 | - |
thymidine | pH 8 | Homo sapiens |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.21 | 25000 | - |
2 * 25000, rLyTK1Val-106 | Homo sapiens |
| 2.7.1.21 | 25000 | - |
4 * 25000, rLyTK1Met-106 | Homo sapiens |
| 2.7.1.21 | 50000 | - |
rLyTK1Val-106, gel filtration | Homo sapiens |
| 2.7.1.21 | 100000 | - |
rLyTK1Met-106, gel filtration | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.1.21 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.1.21 | rLyTK1Val-106 and rLyTK1Met-106 | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.7.1.21 | lymphocyte | healthy and leucemic cells | Homo sapiens | - |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.7.1.21 | dimer | 2 * 25000, rLyTK1Val-106 | Homo sapiens |
| 2.7.1.21 | tetramer | 4 * 25000, rLyTK1Met-106 | Homo sapiens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.21 | 15 | - |
rLyTK1Met-106: 50% loss of activity within 41 min, rLyTK1Val-106: 50% loss of activity after 392 min | Homo sapiens |
| 2.7.1.21 | 25 | - |
50% loss of activity within 4 min, rLyTK1Met-106 | Homo sapiens |
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