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Literature summary extracted from

  • Iwasuki, N.
    Purification and properties of deoxythymidine kinase induced by bacteriophage T4 infection (1977), J. Biochem., 82, 1347-1359.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.21 dTTP protects against thermal inactivation Tequatrovirus T4

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.21 ATP non-competitive Tequatrovirus T4
2.7.1.21 Ba2+ 0.29 mM Tequatrovirus T4
2.7.1.21 dTTP inhibition dependent on pH Tequatrovirus T4
2.7.1.21 Mg2+ non-competitive Tequatrovirus T4
2.7.1.21 Zn2+ 1.1 mM and 2.9 mM Tequatrovirus T4

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 additional information
-
 additional information
-
 Tequatrovirus T4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.21 Ca2+ can partially substitute for Mg2+ Tequatrovirus T4
2.7.1.21 Co2+ can partially substitute for Mg2+ Tequatrovirus T4
2.7.1.21 Mg2+
-
 Tequatrovirus T4
2.7.1.21 Mn2+ can partially substitute for Mg2+ Tequatrovirus T4
2.7.1.21 Ni2+ can partially substitute for Mg2+ Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.21 28000
-
 x * 28000, SDS-PAGE Tequatrovirus T4
2.7.1.21 86000
-
 gel filtration Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21 Escherichia coli infected with bacteriophage Tequatrovirus T4

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.21 11
-
-
 Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + thymidine ATP can be substituted by dATP Tequatrovirus T4 ADP + dTMP
-
 ?
2.7.1.21 additional information no phosphorylation of deoxyuridine, 5-halogenated deoxyuridines Tequatrovirus T4 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.21 ? x * 28000, SDS-PAGE Tequatrovirus T4

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.21 50
-
 quick inactivation above, dTTP protects against thermal inactivation Tequatrovirus T4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.21 6.8
-
-
 Tequatrovirus T4

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.21 5 8 60% of maximal activity at pH 5.0, 50% of maximal activity at pH 8.0 Tequatrovirus T4

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.21 2.5
-
 Mg2+ pH 7, 37°C Tequatrovirus T4
2.7.1.21 3.8
-
 ATP pH 7, 37°C Tequatrovirus T4