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Literature summary extracted from

  • Gan, T.E.; Brumley, J.L.; van der Weyden, M.B.
    Human thymidine kinase. Purification and properties of the cytosolic enzyme of placenta (1983), J. Biol. Chem., 258, 7000-7004.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 0.003
-
 thymidine pH 7.4 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.21 cytosol
-
 Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.21 Ca2+ can partially substitute for Mg2+ Homo sapiens
2.7.1.21 Mg2+
-
 Homo sapiens
2.7.1.21 Mn2+ can partially substitute for Mg2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.21 44000
-
 2 * 44000, SDS-PAGE in presence of 2-mercaptoethanol Homo sapiens
2.7.1.21 84000 98000
-
 Homo sapiens
2.7.1.21 92000
-
 gel filtration, sucrose density gradient centrifugation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21 rLyTK1Val-106 and rLyTK1Met-106 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.21 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.21 0.031
-
 liver enzyme Homo sapiens
2.7.1.21 0.094
-
 placenta enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + 5-bromo-2'-deoxyuridine
-
 Homo sapiens ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + 5-diaza-2'-deoxyuridine
-
 Homo sapiens ADP + 5-diaza-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + 5-iodo-2'-deoxyuridine
-
 Homo sapiens ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + thymidine ATP can be substituted by GTP Homo sapiens ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine ATP can be substituted by dGTP Homo sapiens ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine ATP can be substituted by dCTP Homo sapiens ADP + dTMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.21 dimer 2 * 44000, SDS-PAGE in presence of 2-mercaptoethanol Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.21 7.4
-
-
 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.21 Homo sapiens linear pH gradient between pH 7 and pH 11
-
 9.1