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Literature summary extracted from

  • Sitaramayya, A.
    Rhodopsin kinase prepared from bovine rod disk membranes quenches light activation of cGMP phosphodiesterase in a reconstituted system (1986), Biochemistry, 25, 5460-5468.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.11.14 instability of enzyme, protease inhibitors stabilize during purification Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.14 additional information not inhibited by Na+ Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.14 membrane membrane-bound Bos taurus 16020
-
2.7.11.14 membrane rod disc membranes Bos taurus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.14 Mg2+ requirement Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.14 65000
-
 1 * 65000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.14 ATP + rhodopsin Bos taurus
-
 ADP + phosphorhodopsin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.14 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.14 phosphoprotein enzyme is autophosphorylated in the absence of rhodopsin Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.14 partial Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.14 eye retina Bos taurus
-
2.7.11.14 eye dark-adapted Bos taurus
-
2.7.11.14 retina rod cell outer segment Bos taurus
-
2.7.11.14 retina from dark-adapted eyes Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.14 0.062
-
 pH 7.5, 23°C Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.11.14 4°C, highly unstable, partially purified preparation, within 4-5 days, 90% loss of activity, mixture of protease inhibitors stabilizes for several weeks Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.14 ATP + rhodopsin
-
 Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin phosphorylates bovine rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 additional information enzyme quenches light activation of cGMP phosphodiesterase in a reconstituted system Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.14 monomer 1 * 65000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.14 23
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.14 7.5
-
 assay at Bos taurus