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Literature summary extracted from

  • Falke, D.; Nehrbass, W.; Brauer, D.; Müller, W.E.G.
    Adenylic acid: deoxythymidine 5-phosphotransferase: evidence for the existence of a novel Herpes simplex virus-induced enzyme (1981), J. Gen. Virol., 53, 247-255.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.114 0.0021
-
 thymidine 37°C, pH 7.8 Herpes simplex virus
2.7.1.114 0.0023
-
 AMP 37°C, pH 7.8 Herpes simplex virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.114 90600 111000 glycerol density gradient centrifugation Herpes simplex virus
2.7.1.118 75200
-
 virus type 1, glycerol gradient centrifugation Herpes simplex virus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.114 AMP + thymidine Herpes simplex virus
-
 adenosine + dTMP
-
 ?
2.7.1.118 ADP + thymidine Herpes simplex virus
-
 AMP + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.114 Herpes simplex virus
-
 type 1
-
2.7.1.114 Herpes simplex virus
-
 hamster BHK cells infected with virus
-
2.7.1.118 Herpes simplex virus
-
 in hamster BHK-cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.114 type 1, hamster BHK-cells infected with virus Herpes simplex virus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.114 42.2
-
-
 Herpes simplex virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.114 AMP + thymidine
-
 Herpes simplex virus adenosine + dTMP
-
 ?
2.7.1.118 ADP + thymidine
-
 Herpes simplex virus AMP + dTMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.114 37 38
-
 Herpes simplex virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.114 7.6
-
-
 Herpes simplex virus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.114 6.3 8.8 17% of maximal activity at pH 6.3, 32% of maximal activity at pH 8.8 Herpes simplex virus