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Literature summary extracted from

  • Hansen, T.; Schonheit, P.
    Sequence, expression, and characterization of the first archaeal ATP-dependent 6-phosphofructokinase, a non-allosteric enzyme related to the phosphofructokinase-B sugar kinase family, from the hyperthermophilic crenarchaeote Aeropyrum pernix (2001), Arch. Microbiol., 177, 62-69.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 ammonium sulfate 50 mM, 50% inhibition Aeropyrum pernix
2.7.1.11 KCl 100 mM, 50% inhibition Aeropyrum pernix
2.7.1.11 NaCl 1 M, 50% inhibition Aeropyrum pernix

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 0.25
-
 ATP pH 6.7, 85°C Aeropyrum pernix
2.7.1.11 0.6
-
 D-glucose 6-phosphate pH 6.7, 85°C Aeropyrum pernix
2.7.1.11 0.68
-
 D-fructose 6-phosphate pH 6.7, 85°C Aeropyrum pernix
2.7.1.11 1
-
 adenosine pH 6.7, 85°C Aeropyrum pernix
2.7.1.11 1
-
 fructose pH 6.7, 85°C Aeropyrum pernix
2.7.1.11 3
-
 D-ribose 5-phosphate pH 6.7, 85°C Aeropyrum pernix
2.7.1.11 5
-
 D-ribose pH 6.7, 85°C Aeropyrum pernix

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 Co2+
-
 Aeropyrum pernix
2.7.1.11 Mg2+ required for activity Aeropyrum pernix
2.7.1.11 Mn2+ 9% of activity with Mg2+ Aeropyrum pernix
2.7.1.11 Ni2+ 15% of activity with Mg2+ Aeropyrum pernix
2.7.1.11 Zn2+ 46% of activity with Mg2+ Aeropyrum pernix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 33392
-
 alpha4, x * 33392, deduced from nucleotide sequence Aeropyrum pernix
2.7.1.11 115000
-
 gel filtration Aeropyrum pernix

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate Aeropyrum pernix
-
 ADP + D-fructose 1,6-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Aeropyrum pernix Q9YG89 hyperthermophilic crenarchaeote
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 recombinant PFK, Bio-Scale Q5, gel filtration Aeropyrum pernix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + adenosine
-
 Aeropyrum pernix ADP + ?
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Aeropyrum pernix ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + fructose
-
 Aeropyrum pernix ADP + fructose 1-phosphate
-
 ?
2.7.1.11 ATP + glucose 6-phosphate
-
 Aeropyrum pernix ADP + glucose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + ribose
-
 Aeropyrum pernix ADP + ribose 1-phosphate
-
 ?
2.7.1.11 ATP + ribose 5-phosphate
-
 Aeropyrum pernix ADP + ribose 1,5-bisphosphate
-
 ?
2.7.1.11 GTP + D-fructose 6-phosphate 76% of activity with ATP Aeropyrum pernix GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ITP + D-fructose 6-phosphate 76% of activity with ATP Aeropyrum pernix IDP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.11 tetramer alpha4, x * 33392, deduced from nucleotide sequence Aeropyrum pernix

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.11 90
-
-
 Aeropyrum pernix

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.11 40 100 very low activity at 40°C and 100°C, approx. 50% of maximal activity at 75°C, activation energy: 57 kJ/mol Aeropyrum pernix

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.11 80
-
 no loss of activity after 2 h Aeropyrum pernix
2.7.1.11 95
-
 complete loss of activity after 2 h Aeropyrum pernix
2.7.1.11 100
-
 complete loss of activity after 30 min, 10% and 45% loss of activity after 30 min in the presence of 1 M KCl and NaCl, respectively Aeropyrum pernix

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.11 6.7
-
-
 Aeropyrum pernix

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.11 5 8.5 60% of maximal activity at pH 5.0 and pH 8.5 Aeropyrum pernix

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.11 0.05
-
 ammonium sulfate pH 6.7, 85°C Aeropyrum pernix
2.7.1.11 0.1
-
 KCl pH 6.7, 85°C Aeropyrum pernix
2.7.1.11 1
-
 NaCl pH 6.7, 85°C Aeropyrum pernix