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Literature summary extracted from

  • Ravi, K.; Rip, J.W.; Carroll, K.K.
    Characterization of dolichol kinase from soybean microsomes (1986), Biochim. Biophys. Acta, 875, 618-625.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.108 Triton X-100
-
 Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.108 CDP
-
 Glycine max
2.7.1.108 CTP
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.108 0.055
-
 dolichol pH 7.4, 37°C Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.108 membrane
-
 Glycine max 16020
-
2.7.1.108 microsome
-
 Glycine max
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.108 Mg2+ low level of stimulation noted, 80% of maximal activity is observed in absence of divalent kcations Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.108 CTP + dolichol Glycine max
-
 CDP + dolichyl phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.108 Glycine max
-
 soybean
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.108 seedling
-
 Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.108 CTP + dolichol
-
 Glycine max CDP + dolichyl phosphate
-
 ?
2.7.1.108 additional information CTP or other phosphate group donors like ATP, GTP or UTP are not required for maximal activity Glycine max ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.108 7 8 second optimum at pH 9.5 Glycine max
2.7.1.108 9.5
-
 second optimum at pH 7.0-8.0 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.108 4 11 no second optimum with Tris-HCl, activity increases fairly linearly between pH 8.0 and 10.0, activity decreases about 25% between pH 9.5 and 10.0 in Chaps buffer Glycine max