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Literature summary extracted from

  • Kanoh, H.; Kondoh, H.; Ono, T.
    Diacylglycerol kinase from pig brain. Purification and phospholipid dependencies (1983), J. Biol. Chem., 258, 1767-1774.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.107 deoxycholate enhances activity Sus scrofa
2.7.1.107 lysophosphatidylcholine activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin Sus scrofa
2.7.1.107 phosphatidylcholine activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin Sus scrofa
2.7.1.107 phosphatidylethanolamine activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin Sus scrofa
2.7.1.107 phosphatidylserine activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin Sus scrofa
2.7.1.107 Phospholipid enhances activity. Activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin Sus scrofa
2.7.1.107 sphingomyelin activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.107 phosphatidate moderate Sus scrofa
2.7.1.107 phosphatidylglycerol inhibits phosphatidylcholine-dependent kinase activity Sus scrofa
2.7.1.107 phosphatidylinositol 2.5 mol% results in 50% inhibition of the phosphatidylcholine-dependent kinase activity Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.107 0.3
-
 ATP pH 7.4, 30°C Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.107 76000
-
 gel filtration Sus scrofa
2.7.1.107 78000
-
 1 * 78000, SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.107
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.107 brain
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.107 8
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.7.1.107 4°C, enzyme concentrated by polyethyleneglycol 6000, stable for 1 week Sus scrofa
2.7.1.107 4°C, purified enzyme, 50% loss of activity after 3 days Sus scrofa

Subunits

EC Number Subunits Comment Organism
2.7.1.107 monomer 1 * 78000, SDS-PAGE Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.107 7.4
-
 phosphatidylcholine-dependent activity Sus scrofa
2.7.1.107 8
-
 broad, deoxycholate-dependent or phosphatidylcholine-dependent activity Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.107 6.5 9 pH 6.5: about 35% of maximal activity, pH 9.0: about 65% of maximal activity, phosphatidylcholine-dependent activity Sus scrofa