Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kato, M.; Takenawa, T.
    Purification and characterization of membrane-bound and cytosolic forms of diacylglycerol kinase from rat brain (1990), J. Biol. Chem., 265, 794-800.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.107 detergent no activity in absence of detergent Rattus norvegicus
2.7.1.107 phosphatidic acid good activator of DGK IV, no effect on DGK I activity Rattus norvegicus
2.7.1.107 phosphatidylcholine moderate enhancement of DGK IV, no effect on DGK I activity Rattus norvegicus
2.7.1.107 phosphatidylinositol enhances activity of DGK I and DGK IV Rattus norvegicus
2.7.1.107 phosphatidylserine enhances activity of DGK I and DGK IV Rattus norvegicus
2.7.1.107 sodium cholate enhances activity of DGK I and DGK IV Rattus norvegicus
2.7.1.107 sodium deoxycholate enhances activity of DGK I and DGK IV Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.107 adenosine 0.5 mM, 5% inhibition of DGK I and 28% inhibition of DGK IV Rattus norvegicus
2.7.1.107 ADP 0.5 mM, 77% inhibition of DGK I and 66% inhibition of DGK IV Rattus norvegicus
2.7.1.107 AMP 0.5 mM, 18% inhibition of DGK I and 14% inhibition of DGK IV Rattus norvegicus
2.7.1.107 ATPgammaS 0.5 mM, 93% inhibition of DGK I and 71% inhibition of DGK IV Rattus norvegicus
2.7.1.107 cAMP 0.5 mM, 20% inhibition of DGK I and 15% inhibition of DGK IV Rattus norvegicus
2.7.1.107 CDP 0.5 mM, 43% inhibition of DGK I and 28% inhibition of DGK IV Rattus norvegicus
2.7.1.107 CTP 0.5 mM, 42% inhibition of DGK I and 9% inhibition of DGK IV Rattus norvegicus
2.7.1.107 GDP 0.5 mM, 74% inhibition of DGK I and 72% inhibition of DGK IV Rattus norvegicus
2.7.1.107 GTP 0.5 mM, 74% inhibition of DGK I and 56% inhibition of DGK IV Rattus norvegicus
2.7.1.107 GTPgammaS 0.5 mM, 37% inhibition of DGK I and 32% inhibition of DGK IV Rattus norvegicus
2.7.1.107 quercetin 0.1 mM, 41% inhibition of DGK I and 15% inhibition of DGK IV Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.107 0.07
-
 1-stearoyl-2-arachidonoyl-sn-glycerol pH 7.4, 30°C, enzyme form DGK I Rattus norvegicus
2.7.1.107 0.09
-
 1,2-diarachidonoyl-glycerol pH 7.4, 30°C, enzyme form DGK I Rattus norvegicus
2.7.1.107 0.09
-
 1-stearoyl-2-arachidonoyl-sn-glycerol pH 7.4, 30°C, enzyme form DGK IV Rattus norvegicus
2.7.1.107 0.1
-
 sn-1,2-dioleoylglycerol pH 7.4, 30°C, enzyme form DGK IV Rattus norvegicus
2.7.1.107 0.125
-
 sn-1,2-dioleoylglycerol pH 7.4, 30°C, enzyme form DGK I Rattus norvegicus
2.7.1.107 0.125
-
 ATP pH 7.4, 30°C, in presence of 1,2-dioleoylglycerol, enzyme form DGK I Rattus norvegicus
2.7.1.107 0.14
-
 1,2-diarachidonoyl-glycerol pH 7.4, 30°C, enzyme form DGK IV Rattus norvegicus
2.7.1.107 0.25
-
 ATP pH 7.4, 30°C, in presence of 1,2-dioleoylglycerol, enzyme form DGK IV Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.107 cytosol DGK I Rattus norvegicus 5829
-
2.7.1.107 membrane bound to Rattus norvegicus 16020
-
2.7.1.107 membrane DGK IV Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.107 Ca2+ DGK I: good activator, DGK IV: activation with 1 mM CaCl2 is comparable to that obtained on presence of MgCl2 Rattus norvegicus
2.7.1.107 Mg2+ DGK I: maximal activation at 20 mM. DGK IV: maximal activation at 5 mM Rattus norvegicus
2.7.1.107 Mn2+ DGK I: activation is not so great as with MgCl2. Maximal stimulation at 0.1 mM. DGK IV: activation is 10fold less than with MgCl2 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.107 DGK I and DGK IV Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.107 brain
-
 Rattus norvegicus
-
2.7.1.107 heart low activity Rattus norvegicus
-
2.7.1.107 kidney low activity Rattus norvegicus
-
2.7.1.107 liver low activity Rattus norvegicus
-
2.7.1.107 lung
-
 Rattus norvegicus
-
2.7.1.107 spleen
-
 Rattus norvegicus
-
2.7.1.107 thymus
-
 Rattus norvegicus
-
2.7.1.107 uterus low activity Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.107 5.187
-
 enzyme form DGK IV Rattus norvegicus
2.7.1.107 11.5
-
 enzyne form DGK I Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-diarachidonoyl-glycerol
-
 Rattus norvegicus ADP + 1,2-diarachidonoyl-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol
-
 Rattus norvegicus ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + sn-1,2-dioleoylglycerol
-
 Rattus norvegicus ADP + sn-1,2-dioleoylglycerol 3-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.107 7.4
-
 DGK I and DGK IV Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.107 6.5 8.5 pH 6.5: about 45% of maximal activity, pH 8.5: about 45% of maximal activity Rattus norvegicus
2.7.1.107 6.8 9 pH 6.5: about 85% of maximal activity pH 9.0: about 50% of maximal activity, enzyme form DGK I Rattus norvegicus