Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Raina, A.; Hyvönen, T.; Eloranta, T.; Voutilainen, M.; Samejima, K.; Yamanoha, B.
    Polyamine synthesis in mammalian tissues. Isolation and characterization of spermidine synthase from bovine brain (1984), Biochem. J., 219, 991-1000.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.16 1,3-diaminopropane 34% inhibition Bos taurus
2.5.1.16 1,6-diaminohexane 23% inhibition Bos taurus
2.5.1.16 5'-ethylthioadenosine
-
 Bos taurus
2.5.1.16 5'-methylthioadenosine non-competitive, mixed type inhibition Bos taurus
2.5.1.16 cadaverine 26-39% inhibition Bos taurus
2.5.1.16 N,N'-Bis-(2-aminoethyl)propane-1,3-diamine strong, 52% inhibition Bos taurus
2.5.1.16 S-Adenosyl(5')-2-methylthioethylamine
-
 Bos taurus
2.5.1.16 spermidine not competitive, mixed type inhibition Bos taurus
2.5.1.16 triethylenetetramine 39% inhibition Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.16 additional information
-
 additional information kinetic study Bos taurus
2.5.1.16 0.0003
-
 S-adenosyl-3-methylthio-1-propylamine pH 7.4, 37ºC Bos taurus
2.5.1.16 0.04
-
 1,4-diaminobutane pH 7.4, 37ºC Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.16 35800
-
 2 * 35800, SDS-PAGE Bos taurus
2.5.1.16 70000
-
 SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Bos taurus
-
 5'-methylthioadenosine + spermidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16 affinity chromatography with S-adenosyl-5'-3-thiopropylamine linked to Sepharose, 11000fold purified Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.16 S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine random order or ordered mechanism in which both substrates bind before the release of the products Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.16 brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.16 1.12
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane analogues of decarboxylated S-adenosylmethionine act as propylamine donors Bos taurus 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,6-diaminohexane 1% the reaction rate than with 1,4-diaminobutane Bos taurus ?
-
 ?
2.5.1.16 S-adenosyl-3-carboxymethylthio-1-propylamine + 1,4-diaminobutane
-
 Bos taurus ?
-
 ?
2.5.1.16 S-adenosyl-3-ethylthio-1-propylamine + 1,4-diaminobutane
-
 Bos taurus ?
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Bos taurus 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane 6% the reaction rate than with 1,4-diaminobutane Bos taurus 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane
-
 ?
2.5.1.16 S-adenosyl-3-propylthio-1-propylamine + 1,4-diaminobutane
-
 Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.16 dimer 2 * 35800, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.16 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.16 additional information
-
 pI 5.22 Bos taurus
2.5.1.16 9.6
-
 190% of the activity, taking pH 7 as 100% Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.16 6.5 9.8 about 60% of maximal activity at pH 6.5 and about 85% of maximal activity at pH 9.8 Bos taurus