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Literature summary extracted from

  • Mukhopadhyay, R.; Shi, J.; Rosen, B.P.
    Purification and characterization of Acr2p, the Saccharomyces cerevisiae arsenate reductase (2000), J. Biol. Chem., 275, 21149-21157.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.20.4.1 GSH required Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.20.4.1 cloned into the bacterial expression vector and expressed in Escherichia coli as a C-terminal histidine-tagged protein Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.20.4.1 arsenite
-
 Saccharomyces cerevisiae
1.20.4.1 phosphate poor Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.20.4.1 0.000002
-
 glutaredoxin glutaredoxin 2 from E. coli Saccharomyces cerevisiae
1.20.4.1 0.0001
-
 glutaredoxin glutaredoxin 3 from E. coli Saccharomyces cerevisiae
1.20.4.1 0.0006
-
 glutaredoxin glutaredoxin 1p from Saccharomyces cerevisiae or glutaredoxin 1 from E. coli Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.20.4.1 16822
-
 2 * 16822, calculation from nucleotide sequence Saccharomyces cerevisiae
1.20.4.1 34000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.20.4.1 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.20.4.1
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.4.1 arsenate + reduced glutaredoxin thioredoxin is unable to support arsenate reduction. The N-terminal Cys residue is essential for arsenate reductase activity. During the catalytic cycle, Acr2p forms a mixed disulfide with GSH before being reduced vby glutaredoxin to regenerate the active Acr2p reductase Saccharomyces cerevisiae arsenite + oxidized glutaredoxin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.20.4.1 dimer 2 * 16822, calculation from nucleotide sequence Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.20.4.1 4.5 6.5
-
 Saccharomyces cerevisiae