BRENDA - Enzyme Database

Characterization of the membrane quinoprotein glucose dehydrogenase from Escherichia coli and characterization of a site-directed mutant in which histidine-262 has been changed to tyrosine

Cozier, G.E.; Salleh, R.A.; Anthony, C.; Biochem. J. 340, 639-647 (1999)
No PubMed abstract available

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.5.2
H262Y
greatly diminished catalytic efficiency for all substrates, rate of electron transfer to oxygen is unaffected, 230fold increased Km value for glucose
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.5.2
1.3
-
6-deoxy-D-glucose
wild-type enzyme
Escherichia coli
1.1.5.2
1.6
-
2-deoxy-D-glucose
wild-type enzyme
Escherichia coli
1.1.5.2
2.1
-
D-glucose
wild-type enzyme
Escherichia coli
1.1.5.2
2.5
-
D-allose
wild-type enzyme
Escherichia coli
1.1.5.2
8.3
-
D-fucose
wild-type enzyme
Escherichia coli
1.1.5.2
9.5
-
2-amino-D-glucose
wild-type enzyme
Escherichia coli
1.1.5.2
10.8
-
3-deoxy-D-glucose
wild-type enzyme
Escherichia coli
1.1.5.2
17.7
-
D-melibiose
wild-type enzyme
Escherichia coli
1.1.5.2
22
-
D-xylose
wild-type enzyme
Escherichia coli
1.1.5.2
32
-
2-deoxy-D-glucose
mutant enzyme H262
Escherichia coli
1.1.5.2
39
-
D-galactose
wild-type enzyme
Escherichia coli
1.1.5.2
46
-
L-arabinose
wild-type enzyme
Escherichia coli
1.1.5.2
78
-
D-mannose
wild-type enzyme
Escherichia coli
1.1.5.2
79
-
3-O-methyl-D-glucose
wild-type enzyme
Escherichia coli
1.1.5.2
100
-
L-Lyxose
wild-type enzyme
Escherichia coli
1.1.5.2
110
-
D-ribose
wild-type enzyme
Escherichia coli
1.1.5.2
460
-
D-glucose
mutant enzyme H262Y
Escherichia coli
1.1.5.2
810
-
D-allose
mutant enzyme H262Y
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.5.2
membrane
-
Escherichia coli
16020
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.5.2
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.5.2
wild-type and mutant enzyme H262Y
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.2
2-amino-D-glucose + pyrroloquinoline quinone
-
639213
Escherichia coli
2-amino-D-glucono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
2-deoxy-D-glucose + pyrroloquinoline quinone
-
639213
Escherichia coli
2-deoxy-D-glucono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
3-deoxy-D-glucose + pyrroloquinoline quinone
-
639213
Escherichia coli
3-deoxy-D-glucono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
3-O-methyl-D-glucose + pyrroloquinoline quinone
-
639213
Escherichia coli
3-O-methyl-D-glucono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
6-deoxy-D-glucose + pyrroloquinoline quinone
-
639213
Escherichia coli
6-deoxy-D-glucono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
D-allose + pyrroloquinoline quinone
-
639213
Escherichia coli
D-allono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
D-fucose + pyrrolquinoline quinone
-
639213
Escherichia coli
6-deoxy-D-galactono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
D-galactose + pyrroloquinoline quinone
-
639213
Escherichia coli
D-galactono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
D-glucose + pyrroloquinoline quinone
-
639213
Escherichia coli
D-glucono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
D-mannose + pyrroloquinoline quinone
-
639213
Escherichia coli
D-mannono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
D-melibiose + pyrroloquinoline quinone
-
639213
Escherichia coli
?
-
-
-
?
1.1.5.2
D-ribose + pyrroloquinoline quinone
-
639213
Escherichia coli
D-ribono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
D-xylose + pyrroloquinoline quinone
-
639213
Escherichia coli
D-xylono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
L-arabinose + pyrroloquinoline quinone
-
639213
Escherichia coli
L-arabino-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
L-lyxose + pyrroloquinoline quinone
-
639213
Escherichia coli
?
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.2
pyrroloquinoline quinone
during the processing of pyrroloquinoline quinone into the apoenzyme to give active enzyme, its affinity is markedly dependent on the pH, four groups with pK values between pH 7 and pH 8 are involved; prosthetic group
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.2
pyrroloquinoline quinone
during the processing of pyrroloquinoline quinone into the apoenzyme to give active enzyme, its affinity is markedly dependent on the pH, four groups with pK values between pH 7 and pH 8 are involved; prosthetic group
Escherichia coli
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.5.2
H262Y
greatly diminished catalytic efficiency for all substrates, rate of electron transfer to oxygen is unaffected, 230fold increased Km value for glucose
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.5.2
1.3
-
6-deoxy-D-glucose
wild-type enzyme
Escherichia coli
1.1.5.2
1.6
-
2-deoxy-D-glucose
wild-type enzyme
Escherichia coli
1.1.5.2
2.1
-
D-glucose
wild-type enzyme
Escherichia coli
1.1.5.2
2.5
-
D-allose
wild-type enzyme
Escherichia coli
1.1.5.2
8.3
-
D-fucose
wild-type enzyme
Escherichia coli
1.1.5.2
9.5
-
2-amino-D-glucose
wild-type enzyme
Escherichia coli
1.1.5.2
10.8
-
3-deoxy-D-glucose
wild-type enzyme
Escherichia coli
1.1.5.2
17.7
-
D-melibiose
wild-type enzyme
Escherichia coli
1.1.5.2
22
-
D-xylose
wild-type enzyme
Escherichia coli
1.1.5.2
32
-
2-deoxy-D-glucose
mutant enzyme H262
Escherichia coli
1.1.5.2
39
-
D-galactose
wild-type enzyme
Escherichia coli
1.1.5.2
46
-
L-arabinose
wild-type enzyme
Escherichia coli
1.1.5.2
78
-
D-mannose
wild-type enzyme
Escherichia coli
1.1.5.2
79
-
3-O-methyl-D-glucose
wild-type enzyme
Escherichia coli
1.1.5.2
100
-
L-Lyxose
wild-type enzyme
Escherichia coli
1.1.5.2
110
-
D-ribose
wild-type enzyme
Escherichia coli
1.1.5.2
460
-
D-glucose
mutant enzyme H262Y
Escherichia coli
1.1.5.2
810
-
D-allose
mutant enzyme H262Y
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.5.2
membrane
-
Escherichia coli
16020
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.5.2
wild-type and mutant enzyme H262Y
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.2
2-amino-D-glucose + pyrroloquinoline quinone
-
639213
Escherichia coli
2-amino-D-glucono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
2-deoxy-D-glucose + pyrroloquinoline quinone
-
639213
Escherichia coli
2-deoxy-D-glucono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
3-deoxy-D-glucose + pyrroloquinoline quinone
-
639213
Escherichia coli
3-deoxy-D-glucono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
3-O-methyl-D-glucose + pyrroloquinoline quinone
-
639213
Escherichia coli
3-O-methyl-D-glucono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
6-deoxy-D-glucose + pyrroloquinoline quinone
-
639213
Escherichia coli
6-deoxy-D-glucono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
D-allose + pyrroloquinoline quinone
-
639213
Escherichia coli
D-allono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
D-fucose + pyrrolquinoline quinone
-
639213
Escherichia coli
6-deoxy-D-galactono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
D-galactose + pyrroloquinoline quinone
-
639213
Escherichia coli
D-galactono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
D-glucose + pyrroloquinoline quinone
-
639213
Escherichia coli
D-glucono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
D-mannose + pyrroloquinoline quinone
-
639213
Escherichia coli
D-mannono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
D-melibiose + pyrroloquinoline quinone
-
639213
Escherichia coli
?
-
-
-
?
1.1.5.2
D-ribose + pyrroloquinoline quinone
-
639213
Escherichia coli
D-ribono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
D-xylose + pyrroloquinoline quinone
-
639213
Escherichia coli
D-xylono-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
L-arabinose + pyrroloquinoline quinone
-
639213
Escherichia coli
L-arabino-1,5-lactone + pyrroloquinoline quinol
-
-
-
?
1.1.5.2
L-lyxose + pyrroloquinoline quinone
-
639213
Escherichia coli
?
-
-
-
?