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Literature summary extracted from

  • Schroeder, G.; Eichel, J.; Breinig, S.; Schroeder, J.
    Three differentially expressed S-adenosylmethionine synthetases from Catharanthus roseus: molecular and functional characterization (1997), Plant Mol. Biol., 33, 211-222.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.6 three isoenzymes expressed in Escherichia coli Catharanthus roseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.6 5-amino-L-norvaline 10-25% inhibition with 5 mM; 10-25% inhibition with 5 mM; 10-25% inhibition with 5 mM Catharanthus roseus
2.5.1.6 ADP 35-50% inhibition with 5 mM; 35-50% inhibition with 5 mM; 35-50% inhibition with 5 mM Catharanthus roseus
2.5.1.6 CTP 60-70% inhibition with 5 mM; 60-70% inhibition with 5 mM; 60-70% inhibition with 5 mM Catharanthus roseus
2.5.1.6 Ethionine 32-38% inhibition with 5 mM; 32-38% inhibition with 5 mM; 32-38% inhibition with 5 mM Catharanthus roseus
2.5.1.6 GTP not accepted as a substrate but inhibits the reaction in the presence of ATP, 70-80% inhibition with 5 mM; not accepted as a substrate but inhibits the reaction in the presence of ATP, 70-80% inhibition with 5 mM; not accepted as a substrate but inhibits the reaction in the presence of ATP, 70-80% inhibition with 5 mM Catharanthus roseus
2.5.1.6 additional information addition of reducing agents has no effect; addition of reducing agents has no effect; addition of reducing agents has no effect Catharanthus roseus
2.5.1.6 putrescine 15-25% inhibition with 5 mM; 15-25% inhibition with 5 mM; 15-25% inhibition with 5 mM Catharanthus roseus
2.5.1.6 spermidine 15-34% inhibition with 5 mM; 15-34% inhibition with 5 mM; 15-34% inhibition with 5 mM Catharanthus roseus
2.5.1.6 spermine 30-40% inhibition with 5 mM; 30-40% inhibition with 5 mM; 30-40% inhibition with 5 mM Catharanthus roseus
2.5.1.6 tripolyphosphate strong inhibitor; strong inhibitor; strong inhibitor Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.6 0.1
-
 L-methionine
-
 Catharanthus roseus
2.5.1.6 0.18
-
 ATP
-
 Catharanthus roseus
2.5.1.6 0.25
-
 ATP
-
 Catharanthus roseus
2.5.1.6 0.31
-
 ATP
-
 Catharanthus roseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.6 K+ monovalent cations required for optimal activity, 50 mM K+ sufficient for full activity, can be replaced by NH4+ but not by Na+ Catharanthus roseus
2.5.1.6 Mg2+ strictly dependent on divalent cations with maximum activity at 5mM and Mg2+ fully replaceable by Mn2+ or Co2+ salts Catharanthus roseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.6 42560
-
 SAMS 3, gel filtration Catharanthus roseus
2.5.1.6 43000
-
 SAMS 2, gel filtration Catharanthus roseus
2.5.1.6 43050
-
 SAMS 1, gel filtration Catharanthus roseus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.6 Catharanthus roseus Q96551 SAMS1; Madagascar perwinkle, L. G. Don, line CP3a, three isoenzymes: SAMS 1, 2 and 3
-
2.5.1.6 Catharanthus roseus Q96552 SAMS2; Madagascar perwinkle, L. G. Don, line CP3a, three isoenzymes: SAMS 1, 2 and 3
-
2.5.1.6 Catharanthus roseus Q96553 SAMS3; Madagascar perwinkle, L. G. Don, line CP3a, three isoenzymes: SAMS 1, 2 and 3
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.6 ATP + L-methionine + H2O
-
 Catharanthus roseus S-adenosyl-L-methionine + phosphate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.6 monomer gel filtration Catharanthus roseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.6 37
-
 assay at Catharanthus roseus
2.5.1.6 37 45
-
 Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.6 7 8.3 three isoenzymes Catharanthus roseus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.6 0.035
-
 tripolyphosphate
-
 Catharanthus roseus