EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.275 | cytosol | - |
Carum carvi | 5829 | - |
4.2.3.20 | soluble | - |
Carum carvi | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.3.20 | additional information | divalent metal ion required, probably Mn2+ | Carum carvi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.275 | (+)-trans-carveol + NAD+ | Carum carvi | enzyme catalyses the final step of (+)-carvone biosynthesis, which is developmentally regulated, key enzyme of the pathway is limonene-6-hydroxylase | (+)-carvone + NADH | - |
? | |
1.14.14.53 | (+)-limonene + NADPH + H+ + O2 | Carum carvi | intermediate in biosynthesis of (+)-carvone | (+)-trans-carveol + NADP+ + H2O | - |
? | |
4.2.3.20 | geranyl diphosphate | Carum carvi | - |
(+)-(R)-limonene + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.275 | Carum carvi | - |
var. Karzo | - |
1.14.14.53 | Carum carvi | - |
var Karzo, annual caraway, var Beija, biennial caraway | - |
4.2.3.20 | Carum carvi | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.2.3.20 | geranyl diphosphate = (R)-limonene + diphosphate | forms the first step of carvone biosynthesis in caraway | Carum carvi |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.275 | fruit | - |
Carum carvi | - |
1.1.1.275 | additional information | developmental regulation, overview | Carum carvi | - |
1.14.14.53 | fruit | - |
Carum carvi | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.275 | (+)-trans-carveol + NAD+ | best substrate | Carum carvi | (+)-carvone + NADH | - |
? | |
1.1.1.275 | (+)-trans-carveol + NAD+ | stereospecificity | Carum carvi | (+)-carvone + NADH | - |
? | |
1.1.1.275 | (+)-trans-carveol + NAD+ | enzyme catalyses the final step of (+)-carvone biosynthesis, which is developmentally regulated, key enzyme of the pathway is limonene-6-hydroxylase | Carum carvi | (+)-carvone + NADH | - |
? | |
1.1.1.275 | (-)-cis-carveol + NAD+ | 65% activity compared to (+)/(-)-trans-carveol | Carum carvi | (-)-carvone + NADH | - |
? | |
1.1.1.275 | (-)-trans-carveol + NAD+ | 5% activity compared to (+)/(-)-trans-carveol | Carum carvi | ? | - |
? | |
1.1.1.275 | (-)-trans-carveol + NAD+ | product identification not possible, below detection limit of GC-MS | Carum carvi | ? | - |
? | |
1.1.1.275 | (-)/(+)-cis-carveol + NAD+ | - |
Carum carvi | (-)-carvone + NADH | - |
? | |
1.14.14.53 | (+)-limonene + NADPH + H+ + O2 | high regio- and stereospecificity, requiring NADPH | Carum carvi | (+)-trans-carveol + NADP+ + H2O | 97% of total product, 2.5% (-)-trans-carveol, 0.5% (-)-cis-carveol | ? | |
1.14.14.53 | (+)-limonene + NADPH + H+ + O2 | intermediate in biosynthesis of (+)-carvone | Carum carvi | (+)-trans-carveol + NADP+ + H2O | - |
? | |
1.14.14.53 | (-)-limonene + NADPH + H+ + O2 | hydroxylation at 10% the rate of (+)-limonene | Carum carvi | (-)-cis-carveol + NADP+ + H2O | - |
? | |
4.2.3.20 | geranyl diphosphate | - |
Carum carvi | (+)-(R)-limonene + diphosphate | - |
? | |
4.2.3.20 | geranyl diphosphate | - |
Carum carvi | (+)-(R)-limonene + diphosphate | high stereoselectivity, 98.4% of (+)-product | ? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.275 | 30 | - |
assay at | Carum carvi |
1.14.14.53 | 30 | - |
assay at | Carum carvi |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.275 | 10 | - |
about | Carum carvi |
4.2.3.20 | 7.5 | - |
- |
Carum carvi |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.275 | NAD+ | absolutely dependent on, NADP+ cannot substitute for NAD+ and cannot act synergistically | Carum carvi | |
1.14.14.53 | NADP+ | - |
Carum carvi |