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Literature summary extracted from

  • Sagami, H.; Morita, Y.; Ogura, K.
    Purification and properties of geranylgeranyl-diphosphate synthase from bovine brain (1994), J. Biol. Chem., 269, 20561-20566.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.29 octyl glucoside 0.8%, 1.2fold activation Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.29 (E,E,E)-geranylgeranyl diphosphate 0.005 mM, 50% inhibition, competitive vs. farnesyl diphosphate Bos taurus
2.5.1.29 (Z,E,E)-Geranylgeranyl diphosphate 0.005 mM, approx. 50% inhibition, competitive vs. farnesyl diphosphate Bos taurus
2.5.1.29 3-azageranylgeranyl diphosphate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.29 0.00074
-
 farnesyl diphosphate
-
 Bos taurus
2.5.1.29 0.0008
-
 geranyl diphosphate
-
 Bos taurus
2.5.1.29 0.002
-
 isopentenyl diphosphate
-
 Bos taurus
2.5.1.29 0.033
-
 dimethylallyl diphosphate
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.29 Mg2+ maximal activity at 1 mM Bos taurus
2.5.1.29 Mg2+ required for activity Bos taurus
2.5.1.29 Mn2+ required for activity Bos taurus
2.5.1.29 Mn2+ maximal activity at 1 mM, less effective than Mg2+ Bos taurus
2.5.1.29 Zn2+ maximal activity at 1 mM, approx. 30% of activation with Mg2+ Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.29 37500
-
 x * 37500, tetramer or pentamer, SDS-PAGE Bos taurus
2.5.1.29 150000
-
 Superose 12, gel filtration Bos taurus
2.5.1.29 190000
-
 Superdex 200, gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate Bos taurus
-
 geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate Bos taurus
-
 geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.29 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.29 ammonium sulfate, farnesylmethyl affinity gel Bos taurus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.29 0.098
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Bos taurus geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Bos taurus geranylgeranyl diphosphate + diphosphate exclusive product ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
 Bos taurus geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate
-
 Bos taurus geranylgeranyl diphosphate + 2 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate
-
 Bos taurus geranylgeranyl diphosphate + 2 diphosphate geranylgeranyl diphosphate + farnesyl diphosphate ?

Subunits

EC Number Subunits Comment Organism
2.5.1.29 ? x * 37500, tetramer or pentamer, SDS-PAGE Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.29 0.0012
-
 (Z,E,E)-Geranylgeranyl diphosphate
-
 Bos taurus
2.5.1.29 0.0012
-
 (E,E,E)-geranylgeranyl diphosphate
-
 Bos taurus