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Literature summary extracted from

  • Mendicino, J.
    Sucrose phosphate synthesis in wheat germ and green leaves (1960), J. Biol. Chem., 235, 3347-3352.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.14 D-fructose 6-phosphate inhibitory at high concentrations Triticum aestivum
2.4.1.14 UDP
-
 Spinacia oleracea
2.4.1.14 UDP competitive with UDP-glucose Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.14 3
-
 D-fructose 6-phosphate
-
 Triticum aestivum
2.4.1.14 7.4
-
 UDP-glucose
-
 Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate Triticum aestivum catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
 ?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate Spinacia oleracea catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.14 Spinacia oleracea
-
-
-
2.4.1.14 Triticum aestivum
-
 wheat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.14 partial Triticum aestivum
2.4.1.14 partial Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.14 germ
-
 Triticum aestivum
-
2.4.1.14 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.14 1.1
-
 purified enzyme Triticum aestivum

Storage Stability

EC Number Storage Stability Organism
2.4.1.14 -10°C, 2 months, 10% loss of activity Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.14 additional information fructose or fructose 1-phosphate are not accepted as substrates Triticum aestivum ?
-
 ?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate
-
 Spinacia oleracea UDP + sucrose 6-phosphate
-
 r
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate
-
 Triticum aestivum UDP + sucrose 6-phosphate equilibrium lies far on the product side r
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Triticum aestivum UDP + sucrose 6-phosphate
-
 ?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Spinacia oleracea UDP + sucrose 6-phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.14 37
-
 stable for at least 20 min Triticum aestivum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.14 3.6
-
 UDP
-
 Triticum aestivum